Objective: The objective of the present work was to reduce the start-up time of aerobic granular sludge (AGS) formation using medium-strength sewage and to find the most effective anaerobic, aerobic (oxic), and anoxic (A/O/A) cyclic time distribution over the 6-hour cycle time for the removal of nutrients. Methods: A rectangular sequencing batch reactor (SBR) operated systematically in A/O/A cycle was designed for AGS formation. In order to achieve complete nutrient removal, the anoxic phase (5% of the total cycle duration) was introduced in the SBR cycle. Results: Growth in the biomass profile was observed after 20 days of operation, and complete granulation was achieved within 35 days of operational studies. Regular-shaped, compact granules with visible outlines and clear boundaries predominate inside the system, with an average particle size of 220.19 µm. The A/O/A system achieved average removal efficiencies of 70.02 ± 5.98%, 78.21 ± 18.66%, 60.62 ± 9.69%, and 53.49 ± 8.14% for COD, NH4+-N, TN, and PO43--P, respectively. The reasons for poor COD and PO43--P removal performance were the short anaerobic phase (25% of total cycle duration of 6 hours) and higher biomass concentration. However, the long aerobic phase (60% of total cycle duration of 6 hours) with the anoxic phase achieved outstanding (100%) NH4+-N removal efficiency after the system reached a steady state. SEM results inferred that rod-shaped bacteria, cocci, and filamentous fungi were the dominant microorganisms found within the cultivated granules. Conclusions: Sewage with a high COD loading rate accelerates AGS formation and development within an A/O/A cycle SBR reactor. The rapid granulation and simultaneous removal of organics and nutrients by the A/O/A mode SBR without any support materials provide an alternative for wastewater treatment.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.