Adipose-derived stem cells (ASCs) improve the self-renewal and survival of fat grafts in breast reconstruction after oncology surgery. However, ASCs have also been found to enhance breast cancer growth, and its role in tumor proliferation remains largely elusive. Here, we explored a novel mechanism that mediates hTERT reactivation during ASC-induced tumor growth in breast cancer cells. In this study, we found the proliferative ability of breast cancer cells markedly increased with ASC coculture. To explore the molecular mechanism, we treated cells with anibody/inhibitor and found that the activation of MEK-ERK pathway was triggered in breast cancer cells by SCF secreted from ASCs, leading to the nuclear recruitment of CBP. As a coactivator of hTERT, CBP subsequently coordinated with RFPL-3 upregulated hTERT transcription and telomerase activity. The inhibition of CBP and RFPL-3 abrogated the activation of hTERT transcription and the promotion of proliferation in breast cancer cells with cocultured ASCs in vitro and in vivo. Collectively, our study findings indicated that CBP coordination with RFPL-3 promotes ASC-induced breast cancer cell proliferation by anchoring to the hTERT promoter and upregulating telomerase activity, which is activated by the MAPK/ERK pathway.

Mammary gland microbiota is closely related to the progression of breast cancer (BC). Recent studies have shown that Listeria is differentially enriched in breast cancer and normal paracancerous breast tissue, and associated with epithelial-mesenchymal transition (EMT) in breast cancer. However, studies have rarely reported the relationship between Listeria monocytogenes (L. monocytogenes) enrichment and clinical parameters and possible mechanisms in breast cancer. In this study, we found that L. monocytogenes was highly enriched in the cytoplasm of cells, especially in tumor tissues. By analyzing the relationship between L. monocytogenesenrichment and the clinicopathological indicators and prognostic survival of patients, we found that L. monocytogenes is positively correlated with BC proliferation, metastasis, and poor recurrence-free survival (RFS) on patients. Moreover, we performed cell proliferation and invasion assays on breast cancer cells bearing L. monocytogenes, and proved that L. monocytogenes was able to promote the proliferation and invasion of breast cancer cells, their proliferation and invasion capacity was significantly reduced after antibiotic treatment. Mechanistically, L. monocytogenes was identified to increase the survival rate of circulating breast cancer cells by implementing the fluid shear stress model. In conclusion, our study demonstrated that L. monocytogenes could promote breast cancer development by increasing survival rate of circulating cancer cells.

Background This study aimed to explore the value of mixed reality (MR) in sentinel lymph node biopsy (SLNB) in patients with breast cancer. Methods A total of 300 patients with breast cancer who underwent SLNB in the Department of Breast Surgery of The Second Affiliated Hospital between August 2018 to March 2019 were enrolled. They were randomly divided into two groups using a random number table. In group A, only dye (an injection of methylene blue) was used to detect sentinel lymph nodes, while in group B MR was used for positioning in addition to dye. Results During surgery, the detection time in group B was significantly shorter than in group A (3.62 ± 1.20 vs.7.87 ± 1.86; p < 0.001). At the one- and six-month post-surgery follow-ups, the incidences of pain and numbness in group B were lower than in group A (2.70% vs. 8.28%, p = 0.036; 0.68% vs. 3.45%, p = 0.094, respectively), the incidence of upper limb dysfunction in group B was lower than that of group A (2.03% vs. 8.97%, p = 0.009; 0% vs. 2.07%, p = 0.120, respectively), and the patient satisfaction score of group B was higher than that of group A (4.04 ± 0.91 vs. 3.32 ± 0.94, p < 0.001). Conclusion MR can construct a three-dimensional visualization model for use in surgery. The application of this technology to SLNB in patients with breast cancer can significantly reduce the detection time and the occurrence of complications and improve patient satisfaction.

Objective To explore the value of mixed reality (MR) in sentinel lymph node biopsy (SLNB) in patients with breast cancer. Methods A total of 300 patients with breast cancer who underwent SLNB enrolled and were randomly divided into two groups. In group A, only dye (an injection of methylene blue) was used to detect sentinel lymph nodes, while in group B MR was used for positioning in addition to dye. (MR localization method: Before the surgery, we built a 1:1 3D reconstruction model based on the patient's CT or MRI original data, and after the patient was injected with dye, we completed MR localization by overlapping the pre-marked image with the model.) Results During surgery, the detection time in group B was significantly shorter than in group A (3.62 ± 1.20 vs.7.87 ± 1.86; p \ 0.001). At 1-month post-surgery follow-up, the incidence of pain in group B was lower than that in group A (2.70 vs. 8.28%, p = 0.036). The incidence of upper limb dysfunction was lower in group B than in group A (2.03 vs. 8.97%, p = 0.009). In terms of the incidence of pain, group B was better than group A (0.68 vs. 3.45%, p = 0.094). The satisfaction of the two groups was scored, and the results showed that group B was better than group A (4.04 ± 0.91 vs.3.32 ± 0.94, p \ 0.001). ConclusionThe application of MR to SLNB in breast cancer can significantly reduce the detection time and the occurrence of complications and improve patient satisfaction.Lin Tao and Zhenchu Feng are contributed equally to this study.