Pectate lyases (Pels) can be used in the textile industrial process for cotton scouring and ramie degumming, and its hydrolyzed products oligo galacturonic acid, are high-value added agricultural and health products. In our previous studies, an alkaline pectate lyase PEL168 mutant, PEL3, was obtained with improved specific activity and thermostability. Here, a facile and rapid method for preparing an immobilized PEL3-inorganic hybrid nanoflower was developed, as it could improve its biocatalytic performance. With 0.02 mg/mL (112.2 U/mL) PEL3 in PBS buffer, five different divalent ions, including Mn 2+ , Ca 2+ , Co 2+ , Zn 2+ , and Cu 2+ , were used as inorganic component. The results showed that PEL3/Cu 3 (PO 4) 2 hybrid nanoflowers presented the highest relative activity with 2.5-fold increase, compared to the free PEL3. X-ray diffraction analysis confirmed that the composition of PEL3/Cu 3 (PO 4) 2 hybrid nanoflowers were pectate lyase PEL3 and Cu 3 (PO 4) 2 •5H 2 O. The optimum temperature and pH of PEL3/Cu 3 (PO 4) 2 hybrid nanoflowers were ascertained to be 55 • C and pH 9.0, respectively, exhibiting subtle difference from the free PEL3. However, the PEL3/Cu 3 (PO 4) 2 hybrid nanoflowers maintained 33% residual activity after 24 h incubation at 55 • C, while the free PEL3 completely lost its activity after 18 h incubation at 55 • C. Furthermore, over 50% residual activity of the PEL3/Cu 3 (PO 4) 2 hybrid nanoflowers was remained, even after four times of repetitive utilization, demonstrating its promising stability for practical application.

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