The Media is a material consisting of a mixture of nutrients used by microorganisms to grow and breed. Molasses and liquid waste know one of the alternative media which has the least inexpensive carbon source and is easily obtained from the waste food industry that is rich in nutrients and minerals, so it has the potential for bacterial growth of Bacillus cereus. The consetration of molasses used for growth medium is 1%; 1.5%; and 2% while liquid waste knows 8%, 10%, and 12%. Cell growth is measured by the method of Spectrophotometry, total plate count (TPC) and biomass while the incubation period of cell growth is measured every 0, 6, 12, 18, and 24 hours. The results showed from the molasses + skim milk and the tofu liquid waste + skim milk, the best medium of bacterial cell growth of B. cereus is a liquid waste know isolates K 12%. While the best growth concentrations molasses Medium bacterial B.cereus was N2% and K2%.
Feliatra, Dessy Yoswaty, Iesje Lukistyowati, and Wahid Hasyimi. 2014. The Potential of the Isolated Probiotics Bacterial from Giant Prawns' Digestive Tract (Macrobrachium Rosenbergii, De Man) with 16S rDNA Sequencing Technique. Aquacultura Indonesiana, 15 (2) : 57-63. Probiotics is a bacterial product that has the advantage for fish; it is not only for growing but also for covering the bacteria's attack. The aim of the study is to determine the types of candidate bacteria's probiotic of which the digestive tract of giant prawns in Teratak Buluh Pekanbaru, Riau. This research was conducted at the Laboratory of Microbiology and Biotechnology of Fisheries and Marine Sciences faculty, University of Riau, Pekanbaru to get a candidate of Probiotic Bacteria and conduct molecular examination that consists of purification of electrophoresis gel and 16S rDNA sequencing at the Institute for Biotechnology Serpong, Tangerang Banten. The method used in this researh was physical, biochemical and molecular test in order to identify the candidate bacteria. The results showed that the are eleven isolates of bacterial are the species Bacillus BDU4 strain from A Bacteria isolates with 99% homology and 10 isolates of bacterial are bacillus genus because the degree of homology below 97% and they are considered as the original indonesia's new species probiotic bacteria.
The purpose of this study is to determine the rate of shoreline change and the level of abrasion in Bukit Batu District, Bengkalis Regency, Riau Province based on several physical variables in the coastal area. End Point Rate (EPR) is a method that can be used to calculate the rate of shoreline change by displaying transects according to their category (high abrasion, medium abrasion, low abrasion, stable, and accretion). From 1991-2021 there was an abrasion of ±139.71 meters equivalent to ±4.65 m/year which mostly occurred in Sukajadi Village and Pangkalan Jambi Village, while accretion of ±27.51 meters equivalent to ±1.58 m/year mostly occurred in Pakning Asal Village and Sejangat Village. Moderate abrasion is the predominant type. Sukajadi and Pangkalan Jambi are villages that have experienced high levels of abrasion. The area affected by abrasion has moderate and fast currents as well as coastal protection in the form of mangroves, most of which have been damaged and require repairs. Therefore, the main factors causing the abrasion are thought to be the lack of coastal protection and high-speed currents and waves flowing from the Malacca Strait.
Abstract. Fibriarti BL, Feliatra, Amin B, Darwis. 2021. Biodegradation of LDPE plastic by local strain of Bacillus sp. isolated from Dump Soil Pekanbaru, Indonesia. Biodiversitas 22: 5484-5490. Plastic is a compound resistant to degradation and takes tens to hundreds of years to decompose completely. The diversity of microbial metabolism allows microbes to degrade plastic and utilize it as a source of energy and carbon for growth. One type of plastic widely used in packaging is LDPE (Low-Density Polyethylene). The purpose of this study was to test the ability of local isolates of Bacillus sp. strains BP4 and BP6 isolated from waste soil and to identify them at molecular level. In LDPE plastic degradation test, pieces of LDPE plastic were added as a carbon source to a mineral solution medium and incubated for 30 days on shaker incubator at 150 rpm. The percentage of plastic degradation was calculated by subtracting the percentage of the initial weight of the plastic with the final weight. The test results showed that two isolates i.e. BP4 and BP6 were able to degrade LDPE plastics with degradation percentages of 7.23 ± 0.64% and 8.19 ± 0.12%, respectively. Molecular identification results revealed that Bacillus sp. strain BP4 showed a similarity of 99.72% with Bacillus anthracis strain X11, while Bacillus sp. strain BP6 showed a similarity of 99.93% with Bacillus paramycoides strain 8929.
The aim of research was to find the molecular characteristics of probiotic bacteria in the digestive tract of grouper fish (Chromileptes altivelis) based on 16S ribosomal DNA technique. The bacteria were isolated from stomach and intestine of the fish and were analized in the Marine Microbiology Laboratory of fishery and marine Science Faculty of Riau University. The bacterial DNA were isolated by using a PCR (polymerase Chain Reaction) and was conducted in the biotechnology laboratory of Diponegoro University, Semarang. The purified DNA was sequenced in the BPPT Tangerang. Result shown that 6 bacterial species may be potential as probiotic. There were Bacillus velesensis strain CR-11, Vibio alginolyticus A3G-2, Bacillus cereus site2S, uncultured bacterium clone BB3S16S-17, Bacillus subtillus strain CICC10066, and Bacillus flexus strain LF-3. these bacteria grow well at pH 2 and this indicated one of probiotic bacteria characteristics.
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