Burkholderia pseudomallei, the cause of the severe disease melioidosis in humans and animals, is a gramnegative saprophyte living in soil and water of areas of endemicity such as tropical northern Australia and Southeast Asia. Infection occurs mainly by contact with wet contaminated soil. The environmental distribution of B. pseudomallei in northern Australia is still unclear. We developed and evaluated a direct soil B. pseudomallei DNA detection method based on the recently published real-time PCR targeting the B. pseudomallei type III secretion system. The method was evaluated by inoculating different soil types with B. pseudomallei dilution series and by comparing B. pseudomallei detection rate with culture-based detection rate for 104 randomly collected soil samples from the Darwin rural area in northern Australia. We found that direct soil B. pseudomallei DNA detection not only was substantially faster than culture but also proved to be more sensitive with no evident false-positive results. This assay provides a new tool to detect B. pseudomallei in soil samples in a fast and highly sensitive and specific manner and is applicable for large-scale B. pseudomallei environmental screening studies or in outbreak situations. Furthermore, analysis of the 104 collected soil samples revealed a significant association between B. pseudomallei-positive sites and the presence of animals at these locations and also with moist, reddish brown-to-reddish gray soils.

BackgroundThe soil-dwelling saprophyte bacterium Burkholderia pseudomallei is the cause of melioidosis, a severe disease of humans and animals in southeast Asia and northern Australia. Despite the detection of B. pseudomallei in various soil and water samples from endemic areas, the environmental habitat of B. pseudomallei remains unclear.Methodology/Principal FindingsWe performed a large survey in the Darwin area in tropical Australia and screened 809 soil samples for the presence of these bacteria. B. pseudomallei were detected by using a recently developed and validated protocol involving soil DNA extraction and real-time PCR targeting the B. pseudomallei–specific Type III Secretion System TTS1 gene cluster. Statistical analyses such as multivariable cluster logistic regression and principal component analysis were performed to assess the association of B. pseudomallei with environmental factors. The combination of factors describing the habitat of B. pseudomallei differed between undisturbed sites and environmentally manipulated areas. At undisturbed sites, the occurrence of B. pseudomallei was found to be significantly associated with areas rich in grasses, whereas at environmentally disturbed sites, B. pseudomallei was associated with the presence of livestock animals, lower soil pH and different combinations of soil texture and colour.Conclusions/SignificanceThis study contributes to the elucidation of environmental factors influencing the occurrence of B. pseudomallei and raises concerns that B. pseudomallei may spread due to changes in land use.

Fire regimes in many north Australian savanna regions are today characterised by frequent wildfires occurring in the latter part of the 7-month dry season. A fire management program instigated from 2005 over 24 000 km2 of biodiversity-rich Western Arnhem Land aims to reduce the area and severity of late dry-season fires, and associated greenhouse gas emissions, through targeted early dry-season prescribed burning. This study used fire history mapping derived mostly from Landsat imagery over the period 1990-2009 and statistical modelling to quantify the mitigation of late dry-season wildfire through prescribed burning. From 2005, there has been a reduction in mean annual total proportion burnt (from 38 to 30%), and particularly of late dryseason fires (from 29 to 12.5%). The slope of the relationship between the proportion of early-season prescribed fire and subsequent late dry-season wildfire was ~-1. This means that imposing prescribed early dry-season burning can substantially reduce late dry-season fire area, by direct one-to-one replacement. There is some evidence that the spatially strategic program has achieved even better mitigation than this. The observed reduction in late dry-season fire without concomitant increase in overall area burnt has important ecological and greenhouse gas emissions implications. This efficient mitigation of wildfire contrasts markedly with observations reported from temperate fire-prone forested systems.