3-OH FAs containing 10 to 18 C and a hydroxyl group in third position are characteristic components of Gram-negative bacteria. These compounds were previously used to detect and quantify Gram-negative bacterial communities in various types of samples, from terrestrial, aquatic and atmospheric environments. The relative abundance of 3-OH FAs in soils was recently shown to vary with mean annual air temperature (MAAT) and pH in soils from Mt. Shennongjia (China). In the current study, the concentrations and abundances of 3-OH FAs were determined in soils from altitudinal transects under tropical and temperate climates-Mt. Rungwe (SW Tanzania) and Mt. Majella (Central Italy), respectively. The aim was to (i) examine and compare the distributions of 3-OH FAs in soils from different climatic zones and to (ii) investigate the potential of 3-OH FAs as temperature and pH proxies. When combined with previously published data, a moderate correlation (R 2 0.62) between the summed iso and anteiso to the total amount of normal 3-OH FAs (RIAN index) and pH is obtained. We show

This review summarizes recent developments in conjugation techniques for the synthesis of cell-penetrating peptide (CPP)–drug conjugates targeting cancer cells. We will focus on small organic molecules as well as metal complexes that were used as cytostatic payloads. Moreover, two principle ways of coupling chemistry will be discussed direct conjugation as well as the use of bifunctional linkers. While direct conjugation of the drug to the CPP is still popular, the use of bifunctional linkers seems to gain increasing attention as it offers more advantages related to the linker chemistry. Thus, three main categories of linkers will be highlighted, forming either disulfide acid-sensitive or stimuli-sensitive bonds. All techniques will be thoroughly discussed by their pros and cons with the aim to help the reader in the choice of the optimal conjugation technique that might be used for the synthesis of a given CPP–drug conjugate