Desa Sakatiga terletak di Kecamatan Indralaya Kabupaten Ogan Ilir, Sumatera Selatan, memiliki potensi pengembangan perikanan budidaya. Permasalahan yang sering dialami oleh pembudidaya ikan lele di desa Sakatiga diantaranya tingginya harga pakan dan minimnya pengetahuan masyarakat tentang bahan lokal yang dapat digunakan sebagai bahan baku pakan ikan. Permasalahan tersebut dapat diatasi dengan membuat pakan secara mandiri. Tujuan dari kegiatan ini adalah mengetahui efektivitas pemanfaatan bahan baku lokal sebagai pakan ikan terhadap peningkatan produktivitas budidaya ikan lele (Clarias sp.) di desa Sakatiga, Kecamatan Indralaya, Kabupaten Ogan Ilir, Sumatera Selatan. Metode yang digunakan yaitu dengan penyuluhan dan diskusi, pelatihan pembuatan pakan serta demplot pemanfaatan bahan lokal sebagai bahan baku pakan ikan serta pendampingan teknis oleh tim dan dibantu mahasiswa yang melaksanakan praktik lapang di lokasi mitra. Berdasarkan hasil yang diperoleh menunjukkan adanya peningkatan pengetahuan masyarakat tentang bahan lokal yang dapat digunakan sebagai pakan ikan serta motivasi masyarakat untuk membuat pakan dengan menggunakan bahan baku lokal. Pemanfaatan bahan baku lokal sebagai pakan ikan lele menghasilkan pertumbuhan bobot dan panjang mutlak masing-masing sebesar 9,94 g dan 3,83 cm, laju pertumbuhan harian 2,21%/hari, efisiensi pakan 103,24% dan kelangsungan hidup 97%.

The featherback Chitala sp in Indonesia inhabit riverine of Sumatra, Java and Kalimantan. Featherbacks have been protected as threatened species. This research aims to identify the sequence of Cytochrome C Oxidase Subunit I (COI) gene of mtDNA, and construct the phylogenetic trees between species of featherbacks in South Sumatra. This research was conducted in March–August 2020. The methods used in barcoding species and determining phylogenetic i.e., DNA isolation, DNA amplification using PCR and sequencing of COI gene regions of mtDNA. The COI gene was sequenced by PCR (Polymerase Chain Reaction) with optimum annealing temperature 500C for 30s with 35 cycles. After editting, sequence length of the COI gene of featherbacks was 621 base pairs (bp) nucleotide. Based on BLASTanalysis, specimen CLP2 from PT PLN Indralaya had the highest similarity (99.28%) to C. lopis (Malaysia), then 98.88% to the same species from Kampar River, Riau. Speciemens from Musi River (CLS1, CLS 3) and PT PLN (CLP3) indicated the highest similarity of 95.19% with C. chitala from India. The phylogenetic trees showed that Chitala formed four sub-clusters and it was clearly distant between species C. lopis and C. chitala (bootstrap value = 73).

Motile Aeromonas Septicaemia (MAS) is one of bacterial A. hydrophila disease frequently infecting freshwater fish include catfish (Pangasius sp.). Controling the disease of bacterial use chemical antibiotic will cause environment pollution. The aim of this research was to determine utilization of natural material that contain antibacterial such Leucas lavandulaefolia powder as antibacterial to treat of A. hydrophila infection for catfish. The implementation of research was from September until October 2014 at the Laboratorium Budidaya Perairan, Faculty of Agriculture, Sriwijaya University and Laboratorium UPT Klinik Kesehatan Sriwijaya University. Research method was using Completely Randomized Design (CRD) with five treat and three replications. The catfish was infected by A. hydrophila with clinical feature were (inflamed and hemorrhage) that give addition Leucas lavandulaefolia powder on commercial feed as many as 4%, 6%, 8%, and 10%. The result showed that addition 10 % of Leucas lavandulaefolia powder on commercial feed was the best to treat of A. hydrophila infection for catfish were significant (P<0,05). In vitro test result showed that Leucas lavandulaefolia powder  at a concentration 10% as an antibacterial potential of A.hydrophila with inhibitor zone diameter 0.5 cm, at in vivo test the catfish that gives addition Leucas lavandulaefolia powder on commercial pellet as many as 10% capable hematocrit increase, reducing leucocyte completely, produce recovery percentage 84.44%, the survival rate 76.67%, highest absolute growth of catfish 3.03 g and higest absolute length of catfish 0.89 cm. Keywords : A. hydrophila, catfish, Leucas lavandulaefolia powder

Budidaya udang galah di lahan suboptimal rawa masih mengalami kendala karena tingkat mortalitas yang tinggi pada stadia pascalarva terutama saat penebaran awal di media air rawa. Kondisi perairan rawa yang kurang sesuai untuk budidaya udang galah perlu ditingkatkan daya dukungnya terutama yang berhubungan dengan kemampuan osmoregulasi dan metabolisme pascalarva udang galah, salah satunya berupa penambahan mineral kalsium. Penelitian ini bertujuan untuk menentukan penambahan kadar kalsium terbaik yang dapat menghasilkan sintasan, tingkat kerja osmotik, dan tingkat konsumsi oksigen terbaik pada pascalarva udang galah selama masa adaptasi pergantian media hidup dari media bersalinitas 12 ppt ke media air rawa 0 ppt, selama 10 hari mulai dari udang galah stadia PL1 hingga PL11 dengan penurunan salinitassecara gradual dan persentase volume. Rancangan percobaan yang digunakan yaitu rancangan acak lengkap dengan lima perlakuan penambahan kalsium pada air rawa pengencer salinitas media, yaitu 0 mg.L-1 (A), 25 mg.L-1 (B), 50 mg.L-1 (C), 75 mg.L-1 (D), dan 100 mg.L-1 (E). Parameter yang diamati yaitu tingkat sintasan, tingkat kerja osmotik, tingkat konsumsi oksigen, kadar kalsium tubuh, dan kualitas fisika kimia media adaptasi. Hasil penelitian menunjukkan bahwa penambahan 50 mg.L-1 kalsium (C) dapat meminimalisir tingkat kerja osmotik terendah yaitu 192,90 mOsm.l-1 H2O dan tingkat konsumsi oksigen 2,678 mgO2.g-1.jam-1.. Selain itu, juga dapat menyebabkan kadar kalsium tubuh hingga mencapai 8,029 mg/L dan sintasan yang diperoleh mencapai 94%. Selama penelitian berlangsung parameter fisika kimia media adaptasi (suhu, pH, oksigen terlarut, amonia, dan alkalinitas) masih dalam kondisi yang dapat ditolerir bagi sintasan udang galah.

Marble goby (Oxyeleotris marmorata) in Indonesia, spread across Sumatra, Kalimantan and Papua. The purpose of this study was to utilize a sequence of mitochondrial DNA Cytochrome Oxidase Subunit I gene, to analyze the phylogenetic tree and genetic distance between cultured and captured populations. This research was conducted on March-August 2020. The methods used in barcoding species were DNA isolation, amplification using PCR (Polymerase Chain Reaction) and sequencing of the COI mtDNA gene. The domesticated samples (n=3) were collected from Gandus Fish Seed Center (GFSC), while the wild samples (n=3) were captured from the Musi River in Beruge Village, Babat Toman District, both in Musi Banyuasin Regency. The sequenced COI mtDNA gene fragments were obtained from the PCR method. Three samples performed good PCR results, while the other three didn’t amplify properly. After the editing process, the COI gene sequencing produced a nucleotide length of 610 bp. Based on the BLAST (Basic Local Alignment Search Tool), domesticated marble goby (OMD2, OMD3) was in the same cluster with marble goby from the Musi River (OMS3). The genetic distance indicated that two specimens of domesticated marble goby were 100% identical, while the wild (OMS3) indicated a genetic distance of 0.0066 to domesticated species.