Bipolar electrochemistry (BPE) has been lately explored as a simple, reliable and novel electrochemical technique for the adjustment of various conductive substrates. Herein, BPE is performed to derive both of cathode and anode electrodes for the development of mediatorless/membraneless biofuel cell (BFC). On one hand, a preferable substrate for immobilization of bilirubin oxidase enzyme is prepared based on the electropolymerization of thiophene-3-carboxcylic acid (TCA) on an Au microfilm as a bipolar electrode. The resulted biocathode as novel bioelectrocatalyst offers a high electrocatalytic activity toward direct oxygen reduction reaction (ORR) with onset potential and current density of 0.55 V (vs. Ag/AgCl) and 867 μA cm−2, respectively. On the other hand, another analogous Au bipolar electrode is electroplated through BPE to derive Au nanostructures (AuNSs). This modified Au electrode is utilized as an anodic platform for immobilization of flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) enzyme aimed at electrocatalytic glucose oxidation. The prepared bioanode displays a current density of 2.7 mA cm−2 with onset potential of −0.03 V. Finally, the proposed bioanode and biocacthode in an assembled membraneless glucose/O2 BFC offers a power output of 146 μW cm−2 with open circuit voltage of 0.54 V. This novel BPE method provides disposable electrochemical platforms for design of novel sensors, biosensors or other devices.

This is a brief report on the fabrication of concentric multi-element metallic carpets through a one-pot rotational bipolar electro-engineering procedure. A suspended piece of nickel foam as a bipolar electrode (BPE) is rotated in an aqueous solution containing a ternary mixture of metal ions when sufficient DC potential is applied to driving electrodes. The customizable tools of this art are potential gradient, rotation, and concentration/kinetic polarizations. Creating the multi-element radial gradient is typically tested in a one-pot artistic jewelry electroplating.