RESUMOAvaliou-se a técnica da imunoperoxidase como método auxiliar para a detecção de Mycoplasma hyopneumoniae em suínos naturalmente infectados. Foram colhidos 80 fragmentos de pulmões de 40 animais provenientes de granjas consideradas negativas e 40 de granjas com diagnóstico positivo de pneumonia enzoótica. Com a utilização de soro policlonal específico (IgG de coelho anti-M. hyopneumoniae) observou-se correlação positiva de 77% entre os diagnósticos microscópicos e imunoistoquímicos, enquanto que a correlação entre os diagnósticos macroscópico e imunoistoquímico foi de 49%. Nas granjas consideradas negativas observou-se presença de discreta imunorreação em 22,5% dos casos, o que poderia indicar a existência de reação cruzada com outros microrganismos. Nas granjas com diagnóstico positivo para pneumonia enzoótica a técnica da peroxidase-anti-peroxidase (PAP) revelou diferentes graus de intensidade, variando de fraca imunomarcação até espesso depósito amarronzado no epitélio ou na luz das vias aéreas, ou ainda no interior de macrófagos, com relação direta entre a intensidade das lesões e da imunorreação. A técnica imunoistoquímica possui sensibilidade de 95% e especificidade de 77,5%, podendo ser recomendada como ferramenta auxiliar, rápida e de baixo custo para o diagnóstico de pneumonia enzoótica suína em laboratórios de rotina em histopatologia.

The objective of this study was to determine the storage efficiency and reconditioning of potato cv. Innovator for processing. Tubers were stored at 6, 7 and 8 °C in the dark for 30, 60, 90, 120 and 150 days and reconditioned at 15 °C for 15 days. TRS and RS were reduced as storage temperature increased from 6 + 15 °C to 8 + 15 °C. Regardless of temperature and storage time, sugar contents were suitable for industry; however non-enzymatic browning remained in category 2 for up to 60, 90 and 150 days in tubers stored at 6 + 15 °C, 7 + 15 °C and 8 + 15 °C, respectively. Starch was influenced by storage time, despite the absence of temperature effect. Regardless of the temperature, sprouting started at 90 days, but the growth of shoots was directly proportional to the increase in temperature. The PPO and POD increased at the beginning of storage but did not cause darkening before the frying. Reduction in storage temperature followed by reconditioning did not delay the onset of sprouting, but reduced the size of sprouts, maintained adequate sugar levels, however it promoted non-enzymatic browning.

Bird of Paradise (Strelitzia reginae Banks) has great market potential due to its intrinsic beauty and longer vase life. However, storage and transportation conditions are not always adequate and may lead to negative consequences on postharvest physiology and the quality of these flowers. Thus, this study determined the effect of pulsing treatment containing methyl jasmonate (MeJA) or salicylic acid (SA) in association with sucrose on the postharvest physiology of Bird of Paradise. Two independent experiments were carried out. In the first one, floral stems were treated with 100, 250 and 400 μmol L -1 of MeJA plus 200 g L -1 of sucrose for 24 h. In the second experiment, the treatments consisted of 2, 4 and 6 mmol L -1 of SA in association with 200 g L -1 sucrose for 24 h. After the pulsing treatment, the floral stems of both experiments were kept in containers with water at room temperature. The analyses were performed on days 0 and 7 and the assessment comprised: water uptake rate, transpiration rate, fresh mass loss, electrolyte leakage, peroxidase activity (POD) and phenolic compounds of the bracts and sepals. MeJA and SA reduced the water uptake rate by the stems. SA reduced transpiration rate, loss of fresh mass and phenolic compounds of sepals on day 0. On day 7, MeJA treatment reduced electrolyte leakage and increased POD activity. Otherwise, SA had no effect on electrolyte leakage and reduced POD activity. These results suggest that MeJA and AS act antagonistically on the electrolyte leakage and POD activity which might promote different effects on the postharvest physiology of Bird of Paradise stems, such as adverse patterns of the transpiration rate.Key words: peroxidase; plant growth regulators; Strelitzia reginae; transpiration Metil jasmonato e ácido salicílico na fisiologia pós-colheita de Ave do Paraíso RESUMO A Ave do Paraíso (Strelitzia reginae Banks) tem grande potencial de mercado devido à sua beleza intrínseca e longa vida de vaso. Contudo, as condições de armazenamento e transporte nem sempre são adequadas e podem levar a consequências negativas sobre a fisiologia e qualidade pós-colheita dessas flores. Portanto, este estudo determinou o efeito do pulsing de metil jasmonato (MeJA) ou ácido salicílico (AS) em associação com a sacarose na fisiologia pós-colheita de flores de Ave do Paraíso. Para isso, dois experimentos independentes foram realizados. No primeiro experimento, as hastes florais foram tratadas com 100, 250 e 400 µmol L -1 de MeJA juntamente com 200 g L -1 de sacarose por 24 h. No segundo experimento, os tratamentos consistiram de 2, 4 e 6 mmol L -1 de AS em associação com 200 g L -1 de sacarose por 24 h. Após a aplicação do pulsing, as hastes florais de ambos os experimentos foram armazenadas em recipientes com água e conduzidos a temperatura ambiente. As análises foram realizadas nos dias 0 e 7 e a avaliação compreendeu: taxa de absorção de água, taxa de transpiração, perda de massa fresca, extravasamento de eletrólito, atividade de peroxidase (POD) e compostos fenólico...

This study evaluated the involvement of peroxidase and polyphenoloxidase on the enzymatic browning of okra and the role of catalase in the appearance of chilling injury in cultivars Amarelinho and Mammoth Spineless treated with hot water. Okra fruits from two cultivars were immersed in water at 40°C, during 15 and 30 minutes. The control treatment was not immersed, and all fruits were stored at 5°C. Visual analysis of fruits was carried out and total chlorophyll content and total phenolic compounds were determined. The activity of the enzymes catalase, peroxidase and polyphenoloxidase was analyzed. In all treatments, the cultivar Mammoth Spineless showed higher values of chlorophyll content and soluble phenolic compounds compared to cultivar Amarelinho. For both cultivars, catalase activity was higher at the last days of storage, regardless if the fruits were immersed or not in hot water. However, the catalase activity was higher in 'Mammoth Spineless', which presented the higher tolerance to chilling injury. The fruit skin browning was coincident with the increase on polyphenoloxidase activity and phenolic compounds content. This relationship was not observed for the peroxidase activity. The heat treatment during 15 minutes increased the length of storage of cultivar Amarelinho, and for 30 minutes of the cultivar Mammoth Spineless.

The present study aimed to evaluate the storage fruit potential of sour passion fruit progenies at temperatures of 7, 14 and 25 °C. The fruits were harvested at 30% yellow peel color, selected, sanitized and stored under refrigeration in chambers with relative humidity of 80% (± 5%) for a period of 12 days. The experiment was installed in a factorial scheme (3 × 3 × 4), with three progenies selected in the breeding program (P42, P45 and P49), three storage temperatures (7, 14 and 25 ° C) and four evaluation periods (0, 4, 8 and 12 days after storage). Analyzes of fruit mass, pulp mass, pericarp mass, pulp yield, pericarp thickness, water loss, soluble solids content, titratable acidity and ascorbic acid content were performed. There were significant differences among the studied progenies, P49 showed better traits than the other progenies, mainly regarding the resistance to water loss, the main trait that affects the quality of the fruits. Storage at 14 °C for twelve days is feasible to maintain the quality of sour passion fruit. The temperature of 7 °C inhibits the ripening of the fruits and promotes dehydration, confirming the non-recommendation of storage in this condition.