Colombia exhibits an extraordinarily high species diversity of the subfamily Apinae (honeybees, bumblebees, stingless bees, orchid bees). This fact makes it worthwhile to look for beeswax as biological material produced by these insects and to prove possible applications in technique and human life. We examined for the first time waxes from pollen and honey pots, brood cells, and involucres of different species of the tribes Bombini, Meliponini, and Apini native to South America. Thermal analyses were carried out with a TA Instruments SDT-Q600 and simultaneous differential scanning calorimetry/thermogravimetric analysis with dry nitrogen as purge gas. Temperature scans started at ambient temperature and went up to 120• C. A fourier transform infrared spectroscopy Prestige-21 spectrometer was used to obtain infrared spectra of the waxes. Our results underline that thermal properties and IR spectra of waxes are more similar within the taxonomic groups than between them and are related to the altitude where bees live. This work contributes to the achievement of information that will serve to establish energy mechanisms used by these insects and to set up conservation strategies to protect them.