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Purpose: The purpose of this research was to investigate the music therapy (MT) effect on blooddonation anxiety and vital signs of participants. Materials and Methods: The study is a simple blind, controlled, randomized clinical trial. Participants were allocated by means of randomized controlled sampling. The study was performed in the blood donation centre of an University Hospital in İzmir, Turkey. The study was carried out in 60 participants, 30 randomized to the experimental group and 30 randomized to the control group .The control group was given routine attention, and the experimental group was given an MT intervention. The anxiety levels were measured by means State Anxiety Inventory (SAI) of pre and post questionnaires by a blinded investigator. Results: Vital signs of donors were measured pre and post intervention. There were statistical differences between the control and experimental group in heart rate, breath rate, systolic and diastolic blood pressures measured pre and post intervention. A significant difference was found before and after the application in the anxiety score after MT (p < .05). Conclusions: The music therapy application was accepted and evaluated positively by the participants for decreasing the anxiety of the donors before donation. Including the music therapy to the nurse care, applied routinely for the donors of the blood donation, is recommended. Keywords: Music therapy, blood donation, donor anxiety __________________________________________________________________________________