Purpose:The aim of this research is to analyze Asian and African business students' motivation, family support and culture on business orientation. Design/Methodology/Approach: The research method used is both qualitative and quantitative, where quantitative data is obtained through an online questionnaire of 335 respondents, then the data is supplemented by in-depth interviews with respondents to obtain more complete data. Quantitative data is processed using SPSS software statistical program. Findings: The results showed that descriptively there were differences of Asian and African business students. Based on t-test and F-test, there are positive and significant motivation, family support and culture aspects on business orientation in both groups of students. The results are also in-line with some of similar previous researches where the factor that most influence business orientation is culture. Practical implications: For the university and family business, this study can be useful as one of the materials discussion in the family business class and also design the curriculum. The results of this study shown that, culture have the big contibution on business students orientation. Therefore the univesity and family business can include culture as one of the topic of entrepreneurship curriculum. Originality/Value: This research specifies and focuses on comparative analysis of Asian and African business students, the result can be use for design the business students curriculum, therefore the business motivation indexes of Asian and African can be increase through entrepreneurship education in business schools.