Perfectionism has been suggested as a risk factor for the development of stress and burnout in psychotherapists, but this has not been extensively investigated. This study examined the relationship between perfectionism, stress and burnout in 87 Australian clinical psychologists. Stress had significant influences on the relationship between perfectionism and burnout. Specifically, stress was found to be a partial intervening variable in the relationship between perfectionism and personal burnout. In addition, stress was also found to completely mediate the influence of perfectionism on work-related and client-related burnout. The results indicated that perfectionism was both directly and indirectly related through stress to various types of burnout in clinical psychologists. Implications of the findings for research in to the utility of intervention for high levels of perfectionism in clinical psychologists are discussed.

There has been growing interest in understanding energy metabolism in human embryos generated using assisted reproductive techniques (ART) for improving the overall success rate of the method. Using NMR spectroscopy as a noninvasive tool, we studied human embryo metabolism to identify specific biomarkers to assess the quality of embryos for their implantation potential. The study was based on estimation of pyruvate, lactate and alanine levels in the growth medium, ISM1, used in the culture of embryos. An NMR study involving 127 embryos from 48 couples revealed that embryos transferred on Day 3 (after 72 h in vitro culture) with successful implantation (pregnancy) exhibited significantly (p < 10(-5) ) lower pyruvate/alanine ratios compared to those that failed to implant. Lactate levels in media were similar for all embryos. This implies that in addition to lactate production, successfully implanted embryos use pyruvate to produce alanine and other cellular functions. While pyruvate and alanine individually have been used as biomarkers, the present study highlights the potential of combining them to provide a single parameter that correlates strongly with implantation potential.

Ovarian tissue cryopreservation is the primary treatment modality currently available to women at risk of losing their ovarian function due to cytotoxic therapy. However, the impact of these techniques on the oocyte DNA integrity is not elucidated. Here we have investigated the effect of vitrification and conventional slow freezing of eight week old Swiss albino mouse ovarian tissues on the oocyte and granulosa cell DNA integrity using the comet assay. The intracellular levels of reactive oxygen species in oocytes was measured by 2 0 ,7 0-dichlorodihydrofluorescein diacetate fluorescence. The cryopreservation of ovarian tissue by the slow freezing technique resulted in a significantly higher level of DNA fragmentation in oocytes in comparison to vitrification (p50.05) whereas DNA fragmentation in granulosa cells was significantly higher than the control (p50.01). Further, reactive oxygen species were significantly elevated in oocytes derived from slow freezing when compared to vitrification (p50.05). Therefore, we conclude that the ovarian tissue slow freeze-thawing makes the oocyte and granulosa cells more vulnerable to DNA damage whereas vitrification appears to be a safer method than slow freezing for ovarian tissue cryopreservation.