Flow injection unit with merging zone technique was designed for aniline blue determination spectrophotometrically. This unit consists of a valve was designed for dye injection. The scope of the study is to find the optimum conditions :volume of sample, flow rate of carrier, dead volume, dispersion coefficient and reproducibility. They found 117µl, 4.3 ml/min, zero dead volume, 2.62 and high reproducibility for six times respectively. The range of aniline blue concentration by flow injection analysis was 0. 1 -8 ppm and detection limit 0.1 with R 2 = 0.9952 and 0.01 -16 ppm for spectrophotometric method with R 2 =0.9947 with detection limit 0.01 .

This study proposes an easy and cheap technique to get rid of dangerous Malachite Green (MG)

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