This research aims to determine the nutritional content and the best treatment for the addition of Lemna sp. fermented for the growth of sangkuriang catfish. The research was conducted from February to August 2022, in Aquaculture Laboratory of the Fisheries and Marine Science Faculty, Universitas Padjadjaran. The method used in this research is experimental, and is of a Completely Randomized Design (CRD). It consists of four treatments and four repetitions, namely treatment A (control) treatment B (10% fermented lemna + 90% commercial feed), Treatment C (20% fermented lemna + 80% commercial feed), and Treatment D (30% fermented lemna + 70% commercial feed). Parameters observed were changes in nutrient content of lemna, daily growth rate, feed conversion ratio, survival rate, and water quality. Data were analyzed using ANOVA analysis of variance and if there was a significant difference, Duncan test was performed with a 95% confidence level, while changes in nutrients in lemna and water quality were analyzed descriptively. The results of the proximate analysis showed that the fermented lemna using BIOM-S probiotics experienced changes in the nutritional content, namely an increase in protein content from 25.24% to 33.66% and a decrease in crude fiber from 11.93 to 9.13%. Based on the results of this research, the treatment C (20% fermented lemna + 80% commercial feed) the best results in a daily growth rate at 1.92%/day, a feed conversion ratio at 1.41, and a survival rate of up to 97.5%.