The need for water supply at University X still depends on groundwater. The use of groundwater as a source of water at University X as sanitation for students and academic workers. The purpose of this study is to identify the quality of groundwater in all faculties at the University X with parameters nitrate (NO3-), manganese (Mn), pH, TDS, and Escherichia Coli and to investigate the correlation between distance of wells and septic tank with quality of groundwater especially Escherichia Coli. Chemical parameter of groundwater was analyzed by Standar Nasional Indonesia (SNI) method, and biological parameter by using Most Probable Number method and compared with the regulation Minister of Health of the Republic of Indonesia No. 32, 2017. The research was conducted by taking samples of well by tap water to see physical, chemicals, and biological parameters. Analyzing showed that TDS has no significant number that exceed the regulation standard. Other parameters, such as pH has 18,52% of samples fulfil the regulation standard. Mangan (Mn) has 45.68% of groundwater samples contaminated and Nitrate (No3-) has 12.35%. E. coli parameters has 25-points sample that contaminated the water quality. In conclusion, Final result of statistic analyzing correlation between water quality and distance between septic tank and well indicates has no significant correlation.