There are several impacts of the relationship between stress and work that profoundly affect the quality of life of people both inside and outside of organizations. The main objective of this paper was to identify stressors in the work environment that may be caused by its nature and conditions as interpersonal conflicts between work, personal obligations and the needs of self-realization in work. The sample consisted of 78 employees, 47 women and 31 men, were administered self-report questionnaires to the respondents. The chi-square test (p = 0.757) in relation to gender showed that the sample is adherent. We used factor analysis, where the factors were extracted based on the analysis of the total variance by the method of Principal Component Analysis (PCA) was used and the test of Kaiser-Meyer-Olkin (KMO). The results showed that both men and women have rates above the recommended stress, with specific variations. The women surveyed had higher incidence of physical problems related to stress than men. Physical activity has proven to be an important form of organization reduce or even avoid stress among employees, implementing prevention programs within the workplace.