We propose a method to assess the distribution of plant assemblages along rivers delimiting homogeneous fluvial types using cluster analysis applied to morphological features, quantified with aerial photos and geographic information system software. A stratified random sampling design along the elevational gradient was used to analyze riparian plant species. Multivariate statistics were applied to detect patterns of variation in the species data, and among functional and ecological groups. Cluster analysis identified four fluvial types: headwater, low sinuosity, braided, and incised. Canonical Correspondence Analysis, INdicator SPecies ANalysis, and partial ordinations all suggested that fluvial types were characterized by well-defined indicator species. The differences found by Redundancy Analysis and nonparametric analysis of variance for functional and ecological groups also supported the fluvial type division, showing different distributional trends for annual and woody species, and the greatest ecological distance between braided and headwater types. Based on the ordination results, dividing fluvial types according to morphological features was justified by environmental and floristic differences, although plant species variability was only partially described. Our results illustrated that the fluvial type classification created using this methodology was consistent with natural plant species distribution patterns.

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Eighteen chromosome counts are reported in nine Mediterranean Romulea species. Among them, four species have a wide distribution range (R. bulbocodium, R. columnae, R. ramiflora and R. rollii) and five are narrow central Mediterranean endemics (R. bocchierii, R. ligustica, R. linaresii, R. requienii and R. revelieri). Chromosome numbers range from 2n=3x=27 in one R. columnae accession to 2n= 6x=54 in R. linaresii. Our results only partially agree with previous reports, and the chromosome number records of R. bocchierii, R. ligustica, R. linaresii subsp. linaresii and R. revelieri presented here are completely new. According to our data and contrary to previous statements, x=9 seems to be the basic chromosome number in this group of Mediterranean Romulea. Moreover, a highly significant negative correlation between the chromosome size and ploidy level was found.