The digestibility of dry matter (DM), crude protein (CP), ether extract (EE), and the concentration of digestible energy (DE) and metabolizable energy (ME) of bovine cheek meal (CM), included in 15 and 20% were compared. The bovine cheeks not chemically contaminated, prior to the pre-tanning stage of the hides, were collected, washed, cooked, dried, and ground for their nutritional evaluation. As the CM cannot constitute 100% of the guinea pig diet, to estimate its digestibility the indigestibility coefficients of a reference diet (Barley meal-BM) were used and assuming that the indigestibility of the BM is the same in the diets with 15% and 20% CM the digestibility of CM was estimated. Twelve six-month-old male guinea pigs were used, divided into three groups of four animals per group, housed in individual metabolic cages for the total collection of urine-free feces. The DM, CP and EE digestibility’s of CM-15% and CM-20% were 72.30, 95.71 and 78.34%, and 76.34, 96.03 and 82.37%, and the DE and ME contents were 4089.5, 3353.8 and 4129.0 and 3385.8 (P < 0.05). The results would indicate that the production of CM would be a viable option to obtain a protein meal of high nutritional value for feeding guinea pigs and could totally replace other less available and expensive protein sources, such as fish meal or soybean meal, contributing to sustainable animal production and with environmental decontamination strategies.