A variety of methods exist for the immortalization of B lymphocytes by Epstein-Barr virus due to the simplicity of such techniques to establish cell lines with stable genomic DNA. Two different methods for immortalizing lymphoblastoid cell lines were compared for differences in techniques and materials, time between initiation and immortalization, and success rate of immortalization. An incubation period in Epstein-Barr virus and the use of conditioned media improved immortalization efficiency from 86 to 98% and decreased the time (usually weeks) from culture initiation to cryopreservation. The resulting cell bank was used to produce DNA for genetic studies focusing on the genes involved in non-insulin-dependent diabetes mellitus.

Symposium on Technical Library Techniques, Pittsburgh, September 7, 1943. (See other articles from this symposium in the November issue.)

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became interested as a result of their success in this work and instituted a similar program on Curacao.Consequently, Mr. Eastwood is extremely well versed in such operations and has completely revised the previous edition on basis of operational procedures necessary to set up a soilless culture unit.The topics covered include, as chapter titles indicate, General plant physiology, General types of soilless culture, Water culture, Sand culture, Gravel culture, The nutrient solution, Technical control of the nutrient solution, Technical control of the plant culture, General plant culture, Common detriments special chemicals, and Analysis of the nutrient solution. He also has an excel-