The water quality and periphyton parameters were investigated at 5 designated stations along Ntawogba stream system that lies within the Port Harcourt metropolis located between latitude 4º5” N and 5º 00”N and longitude 6º55” E and 7º 00”E. Sampling was done for dry (January – March 2004) and wet (June – August 2004)seasons using standard procedures. Samples were collected at the designated stations along the water course for physicochemical (temperature, pH, conductivity, alkalinity DO, BOD5, NO3, NH3, PO4, SO4) and periphyton descriptors (species richness, species diversity, abundance, biomass, and productivity). The spatio-temporal differences for the parameters were evaluated and the physicochemical attributes demonstrated spatial and seasonal trends but were essentially not statistically significant while the periphyton descriptors demonstrated seasonal and spatial differences and are more reliably affected by the waste water discharges.