Deinococcus ficus sp. nov., isolated from the rhizosphere of Ficus religiosa L. A pale-pink strain (CC-FR2-10 T ) from the rhizosphere of the sacred tree Ficus religiosa L. in Taiwan was investigated by using a polyphasic taxonomic approach. The cells were Gram-positive, rod-shaped and non-spore-forming. Phylogenetic analyses using the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus Deinococcus, the highest sequence similarities being found with Deinococcus grandis (96?1 %), Deinococcus radiodurans (94?3 %), Deinococcus radiopugnans (93?2 %), Deinococcus indicus (93?0 %), Deinococcus proteolyticus (92?5 %), Deinococcus murrayi (92?4 %) and Deinococcus geothermalis (90?7 %). The DNA-DNA relatedness with respect to D. grandis DSM 3963 T was 17?9 %. Chemotaxonomic data revealed that strain CC-FR2-10 T contains only menaquinone MK-8 as the respiratory quinone, unknown phosphoglycolipids as the predominant polar lipids and 16 : 1v7c, 17 : 1v8c and 17 : 1v9c iso as the predominant fatty acids. The biochemical and chemotaxonomic properties demonstrate that strain CC-FR2-10 T represents a novel species, for which the name Deinococcus ficus sp. nov. is proposed. The type strain is
A yellow-pigmented bacterial strain (CC-H3-2 T ), isolated from the rhizosphere of Lactuca sativa L. (garden lettuce) in Taiwan, was investigated using a polyphasic taxonomic approach. The cells were Gram-negative, rod-shaped and non-spore-forming. Phylogenetic analyses using the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus Chryseobacterium, with the highest sequence similarity to the type strains of Chryseobacterium indoltheticum (97?7 %), Chryseobacterium scophthalmum (97?5 %), Chryseobacterium joostei (97?2 %) and Chryseobacterium defluvii (97?2 %). The major whole-cell fatty acids were iso-C 15 : 0 (52?2 %) and iso-C 17 : 0 3-OH. DNA-DNA hybridization experiments revealed levels of only 27?4 % to C. scophthalmum, 27?1 % to C. indoltheticum, 14?1 % to C. joostei and 7?8 % to C. defluvii. DNA-DNA relatedness and biochemical and chemotaxonomic properties demonstrate that strain CC-H3-2 T represents a novel species, for which the name Chryseobacterium formosense sp. nov. is proposed. The type strain is CC-H3-2 T (=CCUG 49271 T =CIP 108367 T ).
A bacterial isolate obtained from soil from a municipal landfill site in India was characterized using a polyphasic taxonomic approach. The colonies of the isolate were found to be yellow and highly mucoid. Comparative analysis of the 16S rRNA gene sequence showed that this isolate constitutes a distinct phyletic line within the genus Lysobacter, displaying >3 % sequence divergence with respect to recognized Lysobacter species. The generic assignment was confirmed by chemotaxonomic data, which revealed the presence of a fatty acid profile characteristic of members of the genus Lysobacter and consisting of saturated, unsaturated, straight-chain and branched-chain fatty acids as well as iso-C 11 : 0 3-OH as hydroxylated fatty acid, and the presence of an ubiquinone with eight isoprene units (Q-8) as the predominant respiratory quinone. The genotypic and phenotypic data show that strain IMMIB APB-9T merits classification as representing a novel species of the genus Lysobacter, for which the name Lysobacter defluvii sp. nov. is proposed. The type strain is IMMIB APB-9 T (=CCUG 53152 T =DSM 18482 T ).
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