Point the finger: Zinc‐finger proteins are convenient and site‐selective adaptors for targeting specific locations within DNA‐origami structures. Orthogonal targeting of the specific locations in the structures was demonstrated by using two adaptors, and the application of Escherichia coli lysate that contained the adaptor‐fused proteins successfully afforded the expected protein–DNA assembly.
Fluorescent biosensors to detect the bona fide events of biologically important molecules in living cells are increasingly demanded in the field of molecular cell biology. Recent advances in the development of fluorescent biosensors have made an outstanding contribution to elucidating not only the roles of individual biomolecules, but also the dynamic intracellular relationships between these molecules. However, rational design strategies of fluorescent biosensors are not as mature as they look. An insatiable request for the establishment of a more universal and versatile strategy continues to provide an attractive alternative, so-called modular strategy, which permits facile preparation of biosensors with tailored characteristics by a simple combination of a receptor and a signal transducer. This review describes an overview of the progress in design strategies of fluorescent biosensors, such as auto-fluorescent protein-based biosensors, protein-based biosensors covalently modified with synthetic fluorophores, and signaling aptamers, and highlights the insight into how a given receptor is converted to a fluorescent biosensor. Furthermore, we will demonstrate a significance of the modular strategy for the sensor design.
Fingerzeig: Zinkfingerproteine sind Adaptoren, die Positionen in DNA‐Origami‐Strukturen ortsselektiv ansteuern. Zwei solche Adaptoren können spezifische Positionen in orthogonaler Weise ansteuern, was in Escherichia‐coli‐Lysat, das Fusionsproteine der Adaptoren enthielt, zum Aufbau der erwarteten Protein‐DNA‐Struktur angewendet wurde.
The methods applied for assembling proteins of interest on DNA scaffolds were categorized and the recent advance of DNA-binding adaptor mediated assembly of proteins on the DNA scaffolds is discussed.
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