Background: As we know metronidazole is a selective treatment for Trichomoniasis, however, it has many side effects, and, on the other hand, it is important to try finding natural alternative treatments. Objectives: The aim of this study was to investigate the effects of Rosmarinus officinalis plant extract on Trichomonas vaginalis parasites and Candida albicans under laboratory conditions. Methods: In this experimental study, Rosmarinus officinalis methanolic extracts were prepared. The anti-trichomoniasis effect was evaluated at concentrations of 0.1, 0.01, 0.001, 0.0004, 0.0002, and 0.0001 µg/mL in TYIS33 culture media and resulted data were analyzed by ANOVA. Results: The results of this study showed that in the concentration of 0.001 at 4 hours, the parasite was spherical and inactivated, while at concentrations of 0.0004, 0.001, 0.01, and 0.1 in 1 hour. The results of this study showed that Rosmarinus officinalis extract in concentration of 100 µg/mL has an inhibitory effect on Candida albicans, so that the highest inhibitory diameter was 18.3 ± 0.9 mm while two other strains exhibit resistance and no inhibitory zones. Conclusions: The results of this study showed that rosemary extract inhibits the growth of Trichomonas vaginalis and Candida albicans that can be used to treat infections.

Background: Acinetobacter baumannii is a non-fermentative gram-negative bacterium that is common in nosocomial infections. As an opportunistic pathogen in hospitals, it is resistant to a large number of antibiotics and responsible for numerous infections such as bacteremia, pneumonia, meningitis, urogenital infections, and surgical wound infections. Methods: We isolated 10 strains of Acinetobacter baumannii from urine samples of patients referring to Zabol Hospital. Solanum nigrum and Saponaria officinalis plants were harvested from Zabol city, dried in shade, milled, placed in a solvent solution (96% ethanol) for 24 h, and extracted using a rotary machine. The minimum inhibitory concentration (MIC) and the least bactericidal concentration were determined by the microdilution method. Results: The results of the antibiotic resistance testing showed that 100% of the strains were susceptible to antibiotics ceftazidime, gentamycin, and azithromycin while only 16.6% were resistant to ampicillin. The results of antimicrobial activity analysis showed that the least minimum inhibitory concentration of Saponaria officinalis leaves extract was 25 ppm against A. baumannii. Nine strains were inhibited at this concentration and one strain survived at all concentrations. The lowest minimum inhibitory concentration of Solanum nigrum extract was 12.5, with two strains being inhibited at this concentration and seven strains being inhibited at 25. Conclusions: This study showed antimicrobial effects of Solanum nigrum and Saponaria officinalis extracts against A. baumannii. Therefore, they can be proposed as treatments for infections caused by this bacterium.