A greenhouse study was conducted to determine the effects of foliar applications of magnetized, chelated liquid iron fertilizer for increasing the drought tolerance of two legumes. Study objectives were to determine the drought tolerance effects of four treatments on foliar gas exchange, soil moisture, and plant growth for velvet bean (Mucuns pruriens) and soybean (Gylcine max) plants. The four foliage treatments included applications with chelated liquid iron fertilizer (2.5 and 5%) with a conventional boom sprayer, with and without magnets in the spray lines. Physiological measurements were collected before foliar treatments and again after a 24-day deficit irrigation schedule. Physicochemical water properties were measured for each of the foliar treatments. Photosynthesis rates were 5.98, 2.04 and 3.19 µmol/m2/s for the control, non-magnetized and magnetized fertilizer treatments (2.5%), respectively, after completing the deficit irrigation schedule. Instantaneous water use efficiency (IWUE) was 0.60, 0.28 and 1.02 for the control, non-magnetized and magnetized fertilizer treatments (2.5%), respectively, after completing the deficit irrigation schedule. Photosynthesis and IWUE increased 56 and 263% for the magnetized fertilizer treatment (2.5%) compared to the non-magnetized foliar treatment, when averaged across both legume species. Photosynthesis and IWUE increased as electrical conductivity increased and oxidation reduction potential (ORP) decreased in absolute terms. A single foliar application resulted in aberrant physiological responses that are contrary to very widely held plant defense theories involving abiotic stressors. The single application improved the photosynthesis and water use efficiency for water stressed legumes emphasizing the need to better understand the relationships between water quality, plant bioenergetics, and stress physiology. Improved drought tolerance in row crops such as dry beans and soybeans, with a single magnetized fertilizer application, would be cost effective and easily adapted into current cropping systems. Interactions among physicochemical water properties, bioenergetics, plant metabolism, and crop stress physiology need to be further investigated in order to improve the quality of irrigation water to enhance drought tolerance of field crops.

An automated truck wash study was conducted at a large layer hen facility to determine the effectiveness of a modified decontamination system for sanitizing semi-trucks and other farm vehicles. The commercial automated power washing system was modified with a fixed gantry that applied a chlorine dioxide (ClO2) disinfectant rinse as the truck exited the biosecurity facility. The truck decontamination study included the primary study plus one smaller Bacillus atrophaeus spore study, as well as air and water sampling. The goal of the field study was to determine the effectiveness of a two-stage automated decontamination system for sanitizing a large, semi-tractor trailer. The primary study objective was to evaluate two power washing techniques (power wash only with a surfactant or power wash with surfactant and a ClO2 rinse). The second objective was to evaluate the decontamination methods on four coupon materials (glass, painted metal, plastic, rubber) to determine the effectiveness of the two-stage wash system on inoculated coupons. The third objective was to determine the effectiveness of the decontamination methods on coupon locations on the truck (front windshield, middle side of trailer, undercarriage). The fourth objective was to determine the effectiveness of the decontamination methods on coupon surface type (coupons coated with or without synthetic grime). The primary study evaluated 48 decontamination treatments to assess their ability to inactivate the MS2 bacteriophage, which is the viral surrogate selected for the study. The results show that the two-stage decontamination treatments increased log10 reduction of the MS2 phage. Log10 reduction increased an average of 247% and 118% for the non-grimed and grimed coupons, respectively, when comparing the automated wash with and without ClO2 rinse across all locations and material types. The average log10 reduction increased from 0.94 to 1.89 for the automated wash and the automated wash + ClO2 rinse, respectively, for the grimed coupons, across all coupon locations and materials. The average log10 reduction increased from 1.23 to 2.17 for the automated wash without ClO2 and the automated wash + ClO2 rinse, respectively, for the non-grimed coupons, across all coupon locations and materials. These results show that combining the ClO2 disinfectant rinse with the automated power wash increased viral efficacy by an average of one log (grimed coupons). Evaluation of the two-stage tuck decontamination system confirms that combining a power wash with a disinfectant rinse increases the ability of the system to sanitize transport trucks and increase farm biosecurity.

A greenhouse study evaluated the widely held hypothesis that invasive plant species have a quicker or stronger response to environmental stimuli such as magnetized irrigation water treatments. A second study objective was to test whether the polarity of magnetized water affected the responses for invasive and non-invasive plant species. Six invasive and six non-invasive plant species were stimulated by magnetizing the seeds followed by applying several magnetized water treatments to the germinated seeds. The species were taxonomically paired then the seeds were exposed to three magnetic field treatments, planted, and irrigated with three magnetized water treatments for approximately two months. The electrical conductivity, oxidation reduction potential (ORP), pH of the water, and nine plant biometrics were measured, collected, and analyzed. The study hypothesis was validated when the invasive species showed enhanced responses to the magnetized seed and water treatments. The invasive species had increased growth in seven out of the nine growth biometrics when exposed to the magnetized seed and water treatments. The long exposure time for pretreatment of seeds (six days) and extended exposure time of the water treatments on the magnets (20 h) contributed to the higher growth rates. The average increase in foliar biomass and leaf area for two paired, invasive species was 184 and 182%, respectively, for the combined seed/watering treatments. In comparison the average increase in foliar biomass and leaf area for two paired, non-invasive species was 88 and 111%, respectively, for the combined seed/watering treatments. The physicochemical water properties for the three magnetized water treatments were correlated with plant growth. The combined magnetic seed/watering treatments produced growth rates that substantially exceeded crop growth rates in comparable magnetized irrigation studies.