No abstract

and samples were collected. All the samples were evaluated by Haematoxylene-Eosine Stain. This is the first study the groin flap has been used as a xenotransplantation model and successfully preserved. Tissue evaluation indicated inflammation was prominent in the tissue, but these changes were reversible to some extent. We believe further studies are needed, in order to control and modify these changes. A method for composite tissue preservation and a basic model for further investigation has been developed in this study. Additional studies are needed in order to find diverse strategies to modulate the tissue changes.

Purpose: Most studies of facial artery (FA) anatomy have been performed in cadaveric specimens and appear to over estimate the presence of the angular branch. In addition, a less invasive in-vivo technique for assessment would be bene cial for use in preoperative evaluation, especially in reconstructive transplantation. Aim of this study is to evaluate the value of CT angiography in assessment of anatomical variations of the FA and its branches and to determine the true incidence of anatomic variations.Methods: 94 cases with 187 FA were included in this study. Thin-slab maximum intensity projection and volume rendering were used for post processing. FA were classi ed into the following 4 groups; Type-1: short course, terminate proximal to the superior labial artery (LA), Type-2: terminate distal to the superior LA near nasolabial fold, Type-3: classic course, extends to lateral nasal-superior alar or angular branch, Type-4: duplex with dominant lateral angular branch. The anatomy of inferior and superior LA was evaluated separately.

Introduction: Due to the need to ensure the oncologic safety of the patient during mastectomies, skin-sparing flaps are often left too thin for full viability. Partial thickness necrosis is common, occurring in 8-26% of surgeries. It has been shown that the delayed administration of specific active peptidessuch as VEGF-has substantially reduced skin necrosis in rat models. Adenoviral delivery portends greater clinical utility, allowing for sustained production of protein as well as higher and more predictable vector potency. Here we demonstrate the effects of the acute administration of adenoviral VEGF-all and EGR-1 to raised Mcfarlane flaps. Methods:Cranially based McFarlane flaps measuring 9 x 3 cm were raised on the dorsum of male Sprague-Dawley rats. Three intradermal 40 uL vector injections were administered at 10 9 pu along a horizontal plane 4 cm from the base of the flap. adVEGF-all (n=6), adEGR-1 (n=3), adNull (n=5) and Saline (n=5) vectors were administered. A silicone sheet was sutured between the flap and the wound bed, preventing peripheral angiogenesis. Clinical scoring and laser assisted indocyanine green dye angiography (LAICGA, LifeCell SPY-Elite) were performed at POD 0, 1, 2, 3 and 7.