Tungiasis is an ectoparasitic infection caused by the invasion of the skin of a man, dog, pig, or bird with gravid female Tunga species. Prevalence may reach up to 50% in impoverished areas of South America, the Caribbean and Sub-Saharan Africa. Very severe cases characterized by more than 30 embedded sand fleas may be found but are not common. Few studies have been done in Nigeria, and in endemic communities of Lagos, a prevalence as high as 45.2% has been reported. We hereby report a severe case of Tungiasis in Ilaje, an endemic community in the Badagry Local Government area of Lagos during an active surveillance conducted between August 2021 to May 2022 involving Eighteen (18) communities. This case is a 54-year-old male farmer who presented with pain, inflammation, ulceration, and deformation of digits, heels and soles of his limbs. He reported a burning sensation, and pruritus and was observed to have difficulties in walking which was evidenced by a noticeable totter. Embedded fleas in clusters numbering between 30 to 60 per limb were extracted by manual removal and the wounds were treated topically. Tungiasis morbidity often leads to poor health and impoverishment. Although tungiasis is rare in nonendemic areas, however, with the increased travel and opening up of Badagry as a seaport and tourist site, this ectoparasite may further be disseminated if still neglected. It is therefore imperative to investigate the occurrence, create awareness and implementation of preventive measures among local inhabitants. Physicians should be familiarized with this infestation for easy diagnosis and treatment.
When an organism dies, internal nucleases existing within the cells are expected to cause DNA to degrade into smaller fragments over time that will eventually lead to alteration in the profile of the genomic structure of the organism. This study to evaluate the effect of post‐mortem time on the integrity of DNA extracted from the brain and kidney of male Sprague Dawley albino rats.Deoxy ribonucleic acid (DNA) was extracted at different post‐mortem times (0, 24, 48 hours), from the brain and kidney of the male albino rats sacrificed via cervical dislocation. Random amplification of polymorphic DNA Polymerase chain reaction (RAPD‐PCR) was used to detect the effect of post‐mortem time on the integrity of DNA by creating the current profile of the DNA at each post‐mortem time.The post‐mortem DNA profile observed in this study reveals that DNA degradation is a sequential time dependent process.There is a relationship between degradation rate of nuclear DNA and post‐mortem time in the studied organs. The rat brain showed a slower degradation rate and is therefore considered as a valuable organ for studying DNA in longer post‐mortem time. The finding from this study is very useful in forensic science and investigation.
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