STIMULUSundifferentiated sarcoma, one osteogenic sarcoma and one plasmocytoma. Metastases occurred in 3 instances. Among the tumors derived by transplantation there were 63 fibrosarcomas, and a few other miscellaneous growths. Variation in differentiation and growth rate was noted in different generations.Discussion. We have arrived at no conclusion as to the nature of the carcinogenic agent in these experiments. Attention is called to the fact that the procedure described is one of the simplest known for tlhe production of sarcomas. I n this con.nection the production of rat sarcomas ,by Turner3 by embedding disks of bakelite (phenol-formaldehyde) is of interest.In the course of certain surgical procedures, cellophane has been used as a covering or heath,^ and left in the human body. We are not acquainted with any reports of the devel-opment of sarcoma in man subsequent to its use, but this possibility should not be forgotten.Cortclusions. 1. Sarcomas were induced in albino rats by the insertion of regenerated cellulose film either subcutaneously or by wrapping it around one kidney.2. These tumors occurred! in about 35% of the rats surviving the operation more than 1 1 months.3. The tumors were transplantable. 4. This is a simple method for inducing sarcomas experimentally.
.The foregoing results in rats should be taken into consideration in' the surgical use of cellophane in man.In the course of investigations in this laboratory on the characteristics of gastric mucus, various types of stimulation have been studied. These included subcutaneous injection of pilocarpine, gentle mucosal massage, and topical application of a number of aqueous solutions and emulsions including ether (saturated,), ethyl alcohol (SO%), isotonic NaC1 (0.17 N), hypertonic NaCl (0.5 K), clove oil ( 5 % ) , mustard oil ( I % ) , and * A preliminary report of this work was transmitted to the XVII International Physiological Congress (Hollander, F., and Lauber, F. U., Cornmimications X V I I Internat. Plqsiol. Cong., 1947, p. 155). t This investigation was conducted with the aid of grants from the Altman Foundation, and Wyeth, Inc. t The authors wish t o express their thanks to Dr. Sophya Lazard for conducting some of the experiments .distilled ~ater.19~93 Among the several objectives of these investigations was the dliscovery of a mucus-stimulating agent which induces no parietal secretion and therefore might be adopted as a standard stimulus for further work on the physiology of mucus secretion. Of the nine stimuli already studied, the 5% clove oil-water emulsion was clearly the most suitable for the purpose, as evidenced by the following characteristics of specimens obtained after a single application of this agent: (a) high viscosity, (b) high pH,* and ( c ) a large total volume relative
