No abstract

The interest in hazelnut (Corylus avellana L.) cultivation has recently increased in Hungary, it is currently grown on 490 hectares. In August 2021 early powdery mildew symptoms were observed in a hazelnut plantation, and in a variety collection of the Hungarian University of Agricultural and Life Sciences in Érd. White patches of mycelium and conidia were observed on both side of the leaves. In early October abundant chasmothecia were formed. The disease incidence was 100% on varieties 'Segorbe', and 'Corabel', 70% on 'Ennis', and 30% on the leaves of 'Istrska dolgoplodna leska' (15 plants per cultivar). Powdery mildew is usually caused by Phyllactinia guttata, which was present abundantly on the abaxial and sparsely on the adaxial surface of the observed leaves. However, another fungus co-occurred on the adaxial surface of the leaves, and rarely occurred on the abaxial surface of the leaves. Its morphology differed substantially from P. guttata on having smaller chasmothecia, and branched appendages. The new powdery mildew agent was morphologically described. Mycelium was hyaline, branched, septate, thin-walled and smooth, 2.5-3.1 μm wide. Conidiophores measured 22 to 61 × 5.1 to 8.5 (average: 44.1 × 6.5) μm (n = 30), the foot cells were erect, cylindrical, and flexuous. Conidia occurred rarely and were produced single on conidiophores, 19 to 34 × 15 to 24 μm. Chasmothecia were spherical, 74 to 103 (average: 85) μm in diameter (n = 100), single or in groups on both sides of each leaf. Appendages 7 to 15 per chasmothecium, aseptate, straight, sometimes flexuous with a length of 74 to 118 (average: 103) μm (n = 50), and had 3 to 5 times dichotomous branched apices with curved tips. Each chasmothecium contained 3 to 5 asci. Ovoid to subglobose asci measured 43 to 65 × 32 to 54 μm (average: 56 × 43) μm (n = 30). Asci contained 4 to 8 ascospores which were hyaline, ellipsoid, measured 17 to 23 × 11 to 20 (mean: 21 × 15) μm (n = 40) in diameter. Morphological identification was confirmed by molecular analysis of two samples, one from the plantation, and one from the variety collection. After DNA extraction partial rDNA internal transcribed spacer region (ITS) of the isolates was amplified using primers ITS1_F and ITS4_R, as previously indicated (Meparishvili et al. 2019). Obtained sequences were deposited to the GenBank (accession no. OL744964 and OL744961). BLAST analysis indicated that the two samples were showing 100% and 97,81% identity to ITS rDNA sequences of Erysiphe corylacearum from Switzerland (MN822722), and showed low similarity of 83% and 85% each to P. guttata (AB080558). Pathogenicity tests were accomplished on ten healthy two-year-old plants of C. avellana cv. ‘Merveille de Bollwiller’ with the two isolates under controlled environment on 25°C, 80% humidity and 16/8 photoperiod. Plants were artificially inoculated by conidial suspension droplets (104/ml). Symptoms appeared after 7-8 days after inoculation and the developing fungus was morphologically identical to the original isolates. Control plants were treated with distilled water, no symptoms were found on them. E. corylacearum was first observed on C. avellana in Turkey in 2013 (Sezer et al. 2017) and was considered as a highly destructive pathogen. It is also known in neighbouring countries, Ukraine (Heluta et al, 2019), Austria (Voglmayr et al. 2020) and Romania (Rosati et al. 2021). To our knowledge, this is the first report of Erysiphe corylacearum in Hungary.

Sour cherry is one of the most important horticultural crops in the Hungarian market. Its flavour combination makes it ideal for fresh consumption as well as canned products. The Hungarian and European markets have requested for new varieties to be introduced, making the evaluation of breeding and prebreeding material a crucial point. A total of 30 sour cherry accessions from the genebank collection were investigated for their potential inclusion into the breeding program. The main aim of the study was to identify candidates for future breeding programs, selecting their colour profiling and total polyphenolic content (TPC). This study follows the antioxidant activity of cherry species by determining the total content in polyphenols. Polyphenols are found in higher concentration in cherries when compared to other plants and have been identified as free radical scavengers, which are useful to prevent the occurrence of several diseases. Furthermore, TPC has been observed as a contributor of bitterness, acidity, colour, flavour, odour, and oxidative stability. The accessions were evaluated for their colour, fruit weight, flavour profile, firmness, and TPC. Colorimetric data were compared among four methods to illustrate to the Hungarian breeders which of the available approaches is the most accurate for sour cherry breeding. Results suggested that several accessions appear relevant for the breeding program, such as ‘Pipacs 1′, ‘Bosnyák’, ‘Hortenzia Királynője’, and ‘Mogyoródi Kései’. The total polyphenolic content was highest in ‘Pipacs1′ and lowest in ‘Kántorjánosi 3′. ‘Pipacs 1′ and ‘Hortenzia Királynője’ had interestingly high acidity content and light to very light fruit colours. ‘Bosnyák’ had a deep and dark colour with high soluble sugar content. ‘Mogyoródi Kései’ appears to have the biggest fruit. All mentioned varieties will be included in future breeding programs.