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We illustrate recent findings on the upriver propagation of long waves entering the mouth of the Misa River (Senigallia, Italy). Such a microtidal environment has been recently studied to understand river–sea interactions: it has been found that the river forcing dominates over the marine actions in winter, especially during storms. However, upriver wave propagation is not negligible with low-frequency waves propagating upriver for distances of the order of kilometers. With the aim to better understand the behavior of low-frequency waves propagating upriver, the analysis of the present work builds on field data collected by instruments installed close to the mouth and along the final reach of the Misa River: a tide gauge, two hydrometers and an acoustic Doppler sensor. It has been here observed that the tidal forcing (periods of the order of hours/days) is significantly strong at a distance of more than one kilometer from the river mouth, while shorter waves, like seiches (periods of some hours), are less important and are supposed to largely dissipate at the estuary, although their role could be of importance during relatively short events (e.g., floods).

The European regulatory framework requires prior authorization for the use of plants and activities that may impact the environment, to ensure an adequate level of environmental and human health protection. Data on industrial wastewater, urban sewage, atmospheric emissions and waste management of installations subject to licenses generate interest among stakeholders (Environmental Protection Agencies, Police Forces, regions, provinces, municipalities, Local Health Authorities, operators, research bodies, citizens), but unfortunately, they are not properly digitized and processed, as they are often unavailable. Therefore, the main goal of this project is to provide a flexible, interactive and easy-to-use tool for stakeholders to allow them to consult these kinds of environmental data. For this purpose, a database integrated with a web-based Geographical Information System (GIS) platform to collect, manage and share pollutant emissions data, is currently being designed and developed. The web platform offers to users interconnected informative modules on required authorizations, license processes and analytical controls. Moreover, stored data from industries and on emissions are georeferenced and displayed in a web GIS map. Stakeholders have free but limited access to specific sections of the web platform, depending on their tasks. They can consult environmental data and GIS representations, download reports or documents and enter new data. This web application seeks to: (1) promote collaboration among stakeholders, (2) simplify the monitoring of an installation’s authorization status and industrial emissions trends, (3) optimize the environmental licensing procedures, (4) enhance the management of environmental control by the relevant authorities, (5) support health and environmental research, and (6) provide industries with innovative services for data transmission. To evaluate the performance and benefits of the newly-developed tool, a real-life pilot case has been applied using data from industries located in the Province of Ascoli Piceno (Marche region, Italy).

Four mutants of Chlamydomonas reinhardtii with defects in different components of the CO2 concentrating mechanism (CCM) or in Rubisco activase were grown autotrophically at high pCO2 and then transferred to low pCO2, in order to study the role of different components of the CCM on carbon allocation and elemental composition. To study carbon allocation, we measured the relative size of the main organic pools by Fourier Transform Infrared spectroscopy. Total reflection X-ray fluorescence was used to analyze the elemental composition of algal cells. Our data show that although the organic pools increased their size at high CO2 in all strains, their stoichiometry was highly homeostatic, i.e., the ratios between carbohydrates and proteins, lipid and proteins, and carbohydrates and lipids, did not change significantly. The only exception was the wild-type 137c, in which proteins decreased relative to carbohydrates and lipids, when the cells were transferred to low CO2. It is noticeable that the two wild types used in this study responded differently to the transition from high to low CO2. Malfunctions of the CCM influenced the concentration of several elements, somewhat altering cell elemental stoichiometry: especially the C/P and N/P ratios changed appreciably in almost all strains as a function of the growth CO2 concentration, except in 137c and the Rubisco activase mutant rca1. In strain cia3, defective in the lumenal carbonic anhydrase (CA), the cell quotas of P, S, Ca, Mn, Fe, and Zn were about 5-fold higher at low CO2 than at high CO2. A Principle Components Analysis showed that, mostly because of its elemental composition, cia3 behaved in a substantially different way from all other strains, at low CO2. The lumenal CA thus plays a crucial role, not only for the correct functioning of the CCM, but also for element utilization. Not surprisingly, growth at high CO2 attenuated differences among strains.