Background: Long distance practice running are growing and nutritional ergogenic are commonly used as a potential aid in final training and competition performance. Caffeine (CAF) and carbohydrates (CHO) are among the most commonly used supplements due to their expected ergogenic properties that can optimize energetic systems. The objective of this study was to examine potential changes in 10-km running performance with acute isolated and combined CAF and CHO supplementation. Material and method: Fifteen recreational endurance-trained runners performed four 10-km running performance on an official athletic track (400 m) under four supplementation conditions: placebo and placebo (PLA+PLA), placebo and caffeine (PLA+CAF), placebo and carbohydrates (PLA+CHO), caffeine and carbohydrates (CAF+CHO). CAF and CHO supplementation consisted of capsules of 6 mg·kg-1 and 8% CHO solution (1 g·kg-1) respectively, ingested 60 and 30 minutes before the performance tests. Placebo was obtained through empty capsules for CAF and juice for CHO without sugar (Clight®). During each trial running speed to calculate 10-km mean velocity (MV) and maximum heart rate (HRmax) were analyzed. Results: There was a difference in the pacing strategy adopted by the runners with higher MV during the initial phase for PLA+CAF and CAF+CHO groups and in the final phase for PLA+ CHO. However, there was no statistically significant difference in 10-km running performance between the conditions, as well as for HRmax. Conclusions: The use of acute, isolated and combined CAF+CHO supplementation had influence in the pacing strategy, but no in 10- km final performance, of recreational runners.