Several studies have shown that plants of plant origin are more relevant, where the antibacterial and modulating action of antibiotic resistance is highlighted. Turnera subulata is popularly known as "chanana". This plant species is commonly used in Brazilian popular medicine to treat infections and In the search for new alternatives to treat infections caused by resistant microorganisms, many studies. The present study aimed to evaluate the antibacterial and modulating activity of the methyl extract obtained from Turnera subulate alone or in combination with aminoglycoside antibiotics, using the microdilution method. The methyl extract of Turnera subulata was used alone in the antibacterial test, and in combination with antibiotics in the modulation assay. All tests were performed in triplicate. The methyl extract of Turnera subulata presented both antibacterial and antibiotic-modulating effects in vitro, alone or in association with aminoglycosides. The activity of the extract depends on the bacterial strain and may be associated with the presence of tannins and flavonols. However, further studies are required to characterize the potential of Turnera subulata in the development of new drugs against multiresistant bacteria.