Intestinal mucositis is a common complication associated with 5-fluorouracil (5-FU), a chemotherapeutic agent used for cancer treatment. Troxerutin (TRX), a semi-synthetic flavonoid extracted from Dimorphandra gardneriana, has been reported as a potent antioxidant and anti-inflammatory agent. In the present study, we aimed to evaluate the effect of TRX on 5-FU-induced intestinal mucositis. Swiss mice were randomly divided into seven groups: Saline, 5-FU, TRX-50, TRX-100, TRX-150, Celecoxib (CLX), and CLX + TRX-100. The weight of mice was measured daily. After treatment, the animals were euthanized and segments of the small intestine were collected to evaluate histopathological alterations (morphometric analysis), levels of malondialdehyde (MDA), myeloperoxidase (MPO), glutathione (GSH), mast and goblet cell counts, immunohistochemical analysis, and cyclooxygenase-2 (COX-2) activity. Compared to the saline treatment, the 5-FU treatment induced intense weight loss and reduction in villus height. TRX treatment (100 mg/kg) prevented the 5-FU-induced histopathological changes and decreased oxidative stress by decreasing the MDA levels and increasing GSH concentration. TRX attenuated inflammatory process by decreasing MPO activity, intestinal mastocytosis, and COX-2 expression. TRX also reversed the depletion of goblet cells. Our findings suggest that TRX at a concentration of 100 mg/kg had chemopreventive effects on 5-FU-induced intestinal mucositis via COX-2 pathway.

Candida albicans is the most frequently isolated opportunistic pathogen in the female genital tract, with 92.3% of cases in Brazil associated with vulvovaginal candidiasis (VVC). Linalool is a monoterpene compound from plants of the genera Cinnamomum , Coriandrum, Lavandula , and Citrus that has demonstrated a fungicidal effect on strains of Candida spp., but its mechanism of action is still unknown. For this purpose, broth microdilution techniques were applied, as well as molecular docking in a predictive manner for this mechanism. The main results of this study indicated that the C. albicans strains analyzed were resistant to fluconazole and sensitive to linalool at a dose of 256 µg/mL. Furthermore, the increase in the minimum inhibitory concentration (MIC) of linalool in the presence of sorbitol and ergosterol indicated that this molecule possibly affects the cell wall and plasma membrane integrity of C. albicans . Molecular docking of linalool with proteins that are key in the biosynthesis and maintenance of the cell wall and the fungal plasma membrane integrity demonstrated the possibility of linalool interacting with three important enzymes: 1,3-β-glucan synthase, lanosterol 14α-demethylase, and Δ 14-sterol reductase. In silico analysis showed that this monoterpene has theoretical but significant oral bioavailability, low toxic potential, and high similarity to pharmaceuticals. Therefore, the findings of this study indicated that linalool probably causes damage to the cell wall and plasma membrane of C. albicans , possibly by interaction with important enzymes involved in the biosynthesis of these fungal structures, in addition to presenting low in silico toxic potential.

Objetivo: Identificar as principais estratégias preventivas da Tuberculose (TB) na Atenção Primária à Saúde (APS). Métodos: Trata-se de uma Revisão Integrativa da Literatura, em que se buscaram artigos disponíveis nas plataformas de dados: Literatura Latino-americana e do Caribe em Ciências da Saúde, Biblioteca Virtual em Saúde e no Science Direct, em que foram pré-selecionados 136 artigos, dos quais 16 constituíram a amostra final. Os critérios de inclusão foram o período de publicação entre 2014 e 2019, nos idiomas português, inglês e espanhol. Excluíram-se monografias, dissertações e teses, bem como as repetições. Resultados: Indicaram que 12,5% dos artigos relataram a prevenção da TB ainda está predominantemente voltada à vacinação, 12,5% relacionados à profilaxia medicamentosa; apenas 6,25% descreve o Tratamento Diretamente Observado de curta duração; 12,5% destacaram as ações educativas, e 56,25% incluíram a melhoria na estrutura das Unidades Básicas de Saúde(UBS) e qualificação dos profissionais de saúde. Conclusão: Ações preventivas da TB na APS são a imunização, a prevenção secundária através da quimioprofilaxia das pessoas expostas, o tratamento diretamente observado de curta duração, ações educativas, melhoria na descentralização dos serviços de saúde e melhor ênfase da gestão na qualificação dos profissionais e melhoria da infraestrutura das UBS.

This study aimed to evaluate the susceptibility of Staphylococcus aureus strains isolated from the milk of cows with subclinical mastitis to Aloe vera in natura sap, ethanolic extract and in natura gel. The substances were tested using agar well diffusion and agar disk-diffusion methods. There was no inhibition of bacterial growth in the agar disk-diffusion method. On the other hand, the agar well diffusion method displayed in vitro growth inhibition of S. aureus in ethanolic extract and in natura sap of 91.66% (22/24) and 35.29% (6/17), respectively. Although the gel showed no effect against the strains tested in both methods, the agar well diffusion method was the most efficient to evaluate the antimicrobial activity of Aloe vera extracts.

The Fedathi Sequence (SF) is a methodological process whose main focus is the teacher, where it seeks to interact with the student stimulating to think, to reflect on a certain problem situation. This methodology in its development is divided into four phases: “positioning”, “maturation”, “solution”, “proof”, where students will apply the knowledge built to solve the problem situation. At present the discipline of anatomy is rooted in traditional methodology and the sequence becomes a didactic tool to solve traditional teaching problems and promote meaningful learning. An exploratory descriptive study with a quantitative approach was carried out with 40 students of the anatomy discipline. The didactic methodology Fedathi Sequence was used in classes of anatomy of vision, hearing and olfaction. Before and after application of the methodology, a pre‐test and post‐test were performed. After applying the methodology, a semi‐structured questionnaire with 16 questions was used. For statistical analysis, the Mann‐Whitney test was performed, being considered significant when they showed p <0.05. It was observed that 100% of the students liked the Fedathi Sequence method and affirmed that there was learning. There was a statistically significant difference in the number of questions answered in the post test (9.77 ± 0.30) when compared to the pretest (4.0 ± 0.24), demonstrating that there was significant learning. It was verified that 90% of the students would suggest the continuity of the methodology. We found that the Fedathi sequence methodology achieved significant results by promoting sensory system anatomy learning.Support or Funding InformationThis study received funding of CAPES and CNPQThis abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.