Atopic dermatitis (AD) is a chronic inflammatory skin disorder, which is associated with an increased expression of Th2 cytokines with concomitant decrease in IFN-gamma production by circulating CD4+ and CD8+ T cells. The skin of patients with AD is often colonized by Staphylococcus aureus, which may reflect in changes in immunological parameters. The aim of the study was flow cytometric measurement of some peripheral blood lymphocyte subsets expressing naive/memory marker (RA/RO) and activation marker (CD25) as well as intracellular production of IFN-gamma by peripheral blood CD4+ and CD8+ T cells from varied severity AD children and determine the impact of S. aureus skin colonization on cytokines profiles. There was a significant increase in the percentage of CD4+ and CD8+ T cells producing IL-4 and IL-13 and decrease in the percentage of CD4+ and CD8+ T cells producing IFN-gamma upon in vitro stimulation with phorbol 12-myristate 13-acetate and ionomycin in children with AD compared to healthy ones. The absolute number of CD4+ and CD8+ T cells expressing memory marker CD45RO was elevated as compared with controls. The severity of AD was positively correlated with the percentage of lymphocyte subsets: CD45RO+, CD4+CD45RO+, and the percentage of CD3+ and CD4+ expressing CD25 as well as the number of S. aureus on the skin. In conclusion, both CD4+ and CD8+ memory T cells are involved in the immunopathogenesis of AD. S. aureus skin colonization is related with disease severity and changes in expression of CD45RO and CD25 on T cells. A decrease in the percentage of CD4+ and CD8+ T cells producing IFN-gamma in AD children may explain propensity for skin infection.

A significant increase in resistin concentration expressed also as resistin/BMI, and resistin/adiponectin ratios, observed in children with CF may suggests that this adipokine is involved in the inflammatory process underlying the disease and is related to worse spirometric parameters describing airways obstruction.

Increased serum level of APE-12 suggests that this adipokine may be implicated in the pathogenesis of childhood atopic asthma.

There are differences in clinical features of asthma in children with regard to the intensity of esophageal acid exposure. Symptoms of asthma in nonatopic individuals with early onset and difficult-to-control nighttime asthma attacks suggest the possibility of concomitant, clinically relevant GER.

Introduction. Patients with cystic fibrosis (CF) present numerous pathological conditions such as malnutrition, depletion of fat-free mass, metabolic disturbances (abnormal glucose metabolism, increased insulin resistance, chronic energy deficit, local and chronic inflammation), which could affect or be associated with altered adipokines concentration. The aim of the study is to evaluate serum concentration of chemerin, omentin and vaspin in children with cystic fibrosis. Material and Methods. The study group included 21 children (mean age 11.3±0.4 years) with stable CF. The diagnosis of CF was established by sweat test and later confirmed by genetic examination in all subjects. Patients with pulmonary exacerbation were excluded from study. In all children history, physicalexamination, anthropometric parameters, sputum, blood samples, and lung function tests were assessed.The control group consisted of 22 healthy children (mean age 10.9 ±4.4years). Results. The mean serum levels of chemerin and omentin were higher (p<0.001) in CF children than in healthy children. The mean serum vaspin concentration was significantly lower than in controls (p<0.001). Concentrations of examined adipokines showed no correlation with BMI, BMI-SDS, Słowa kluczowe mukowiscydoza, chemeryna, omentyna, waspina, dzieci Streszczenie Wstęp. Pacjenci z mukowiscydozą (cysticfibrosis, CF) prezentują liczne patologiczne stany, jak niedożywienie, zmniejszenie masy tłuszczowej ciała, zaburzenia metaboliczne (zaburzony metabolizm glukozy, wzrost insulinooporności, przewlekły deficyt energii, miejscowe i chroniczne zapalenie), które mogą mieć związek z zaburzeniami stężeń adipokin. Celem badań jest ocena stężenia chemeryny, omentyny i waspiny w surowicy krwi u dzieci z mukowiscydozą. Materiał i metody. Badaniami objęto21 dzieci (średni wiek: 11,7± 1,59 lat) ze stabilną CF. Diagnozę CF u tych dzieci potwierdzono badaniem genetycznym. Pacjentów z zaostrzeniem choroby wykluczono z badań. U wszystkich dzieci przeprowadzono wywiad, badanie fizykalne i antropometryczne, zbadano plwocinę, pobrano krew, wykonano czynnościowe badania płuc. Grupę kontrolną stanowiło 22 zdrowych dzieci (średni wiek: 10,9 ± 4,4 lat). Wyniki. Średnie stężenie chemeryny i omentyny w surowicy krwi było istotnie wyższe (p<0.001), a waspiny znamiennie niższe (p<0,001) u dzieci z mukowiscydozą aniżeli u zdrowych. Nie stwierdzono znamiennych statystycznie korelacji pomiędzy stężeniem badanych adipokin we krwi a BMI, BMI-SDS, glukozą, cholesterolem całkowitym, LDL i HDL we krwi. FEV1 i FVC Praca oryginalna