Birds are hosts for several zoonotic pathogens. Because of their high mobility, especially of longdistance migrants, birds can disperse these pathogens, affecting their distribution and phylogeography. We focused on Borrelia burgdorferi sensu lato, which includes the causative agents of Lyme borreliosis, as an example for tick‐borne pathogens, to address the role of birds as propagation hosts of zoonotic agents at a large geographical scale. We collected ticks from passerine birds in 11 European countries. B. burgdorferi s.l. prevalence in Ixodes spp. was 37% and increased with latitude. The fieldfare Turdus pilaris and the blackbird T. merula carried ticks with the highest Borrelia prevalence (92 and 58%, respectively), whereas robin Erithacus rubecula ticks were the least infected (3.8%). Borrelia garinii was the most prevalent genospecies (61%), followed by B. valaisiana (24%), B. afzelii (9%), B. turdi (5%) and B. lusitaniae (0.5%). A novel Borrelia genospecies “Candidatus Borrelia aligera” was also detected. Multilocus sequence typing (MLST) analysis of B. garinii isolates together with the global collection of B. garinii genotypes obtained from the Borrelia MLST public database revealed that: (a) there was little overlap among genotypes from different continents, (b) there was no geographical structuring within Europe, and (c) there was no evident association pattern detectable among B. garinii genotypes from ticks feeding on birds, questing ticks or human isolates. These findings strengthen the hypothesis that the population structure and evolutionary biology of tick‐borne pathogens are shaped by their host associations and the movement patterns of these hosts.

BackgroundRecently, a new rickettsia named ‘Candidatus Rickettsia vini’ belonging to the spotted fever group has been molecularly detected in Ixodes arboricola ticks in Spain, the Czech Republic, Slovakia and Turkey, with prevalence reaching up to 100 %. The aim of this study was to isolate this rickettsia in pure culture, and to describe it as a new Rickettsia species.MethodsA total of 148 ornitophilic nidicolous ticks Ixodes arboricola were collected in a forest near Breclav (Czech Republic) and examined for rickettsiae. Shell vial technique was applied to isolate rickettsiae in Vero cells. Rickettsial isolation was confirmed by optical microscopy and sequencing of partial sequences of the rickettsial genes gltA, ompA, ompB, and htrA. Laboratory guinea pigs and chickens were used for experimental infestations and infections. Animal blood sera were tested by immunofluorescence assay employing crude antigens of various rickettsiae.ResultsRickettsia vini n. sp. was successfully isolated from three males of I. arboricola. Phylogenetic analysis of fragments of 1092, 590, 800, and 497 nucleotides of the gltA, ompA, ompB, and htrA genes, respectively, showed closest proximity of R. vini n. sp. to Rickettsia japonica and Rickettsia heilongjiangensis belonging to the spotted fever group. Experimental infection of guinea pigs and chickens with R. vini led to various levels of cross-reactions of R. vini-homologous antibodies with Rickettsia rickettsii, Rickettsia parkeri, ‘Candidatus Rickettsia amblyommii’, Rickettsia rhipicephali, Rickettsia bellii, and Rickettsia felis. Laboratory infestations by R. vini-infected I. arboricola larvae on chickens led to no seroconversion to R. vini n. sp., nor cross-reactions with R. rickettsii, R. parkeri, ‘Ca. R. amblyommii’, R. rhipicephali, R. bellii or R. felis.ConclusionsOur results suggest that R. vini n. sp. is possibly a tick endosymbiont, not pathogenic for guinea pigs and chickens. Regarding specific phenotypic characters and significant differences of DNA sequences in comparison to the most closely related species (R. japonica and R. heilongjiangensis), we propose to classify the isolate as a new species, Rickettsia vini.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1742-8) contains supplementary material, which is available to authorized users.

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The Little Owl is currently endangered bird species of agricultural lowland areas in Central Europe. Nesting sites of the Little Owl are often old trees as well as buildings and quarries with suitable nesting cavities. The Little Owl has severely declined in a major part of Europe during the past decades. Information on habitat requirements of the Little Owl and data related to land-use changes at nest sites (covering both the breeding and foraging habitats) are needed for conservation programmes aimed at this bird species. Land-use changes in farmland rank among frequently discussed negative factors causing the population decline of the Little Owl. The aim of this study is to analyse land-use changes at nest sites of the Little Owl in the South-Moravian region (Czech Republic) between the years 1976/1977 and 2014. In both studied periods (1976/1977 and 2014), the most important land-use type within 500 m from the nest sites of the Little Owl was arable land (66.94 % -62.25 %), followed by built-up areas (19.97 % -22.41 %), while the other land-use types made up less than 5 %. The proportion of the particular land-use type did not change significantly between the years 1976/1977 and 2014. The most important change in comparison with the period 1976/1977 was the decrease in the area of arable land by 4.69 % and that of orchards and gardens by 1.99 %, while the surface of built-up areas increased slightly by 2.45 % and that of meadows and pastures by 1.5 %. The analysis shows that at the known nest sites of the Little Owl in the South-Moravian region (Czech Republic), there were no significant changes in the proportion of the particular land-use types within 500 m from the nests between the years 1976/1977 and 2014. Based on these results, we can conclude that in comparison with the availability of nest sites, which seems to be the important limiting factor for the occurrence and population density of the Little Owl, land-use changes in study area were not very important factor influencing decline of the Little Owl.