No abstract

No abstract

activity# on tl silicagel G plate** using EkO-hexane (10:90 v/v) as the solvent. The coenzyme Q area adjacent to a coenzyme &lo reference was scraped from the plate and eluted with EkO. The radioactivity in the residue, after evapn of EtzO, was measured using a Nuclear Chicago liquid scintillation spectrophotometer.I n an identical experiment, the residue contg the isolated coenzymes Qwith the coenzymeQ10 carrier and the appropriate reference coenzymes Q were applied to a Whatman No. 3 MM chromatographic paper impregnated with Dow Corning No. 550 silicone oil. Reversed-phase paper chromatog in n-PrOH-HzO (70:30 v/v), which will sep coenzymes Q with isoprenyl side chain lengths 10 through 7 (1, n = 7), was performed.The coenzymes Q8 and Q, areas from the reversed-phase paper chromatogram were rechromatogd in order to determine if these coenzymes Q were actually present or if the radioactivities in $ Constant specific activity of the coenzymes Q was usually attained after the first tlc sepn. Relative specific activities of the product coenzymes Q were not indicated due to the nature of [Wlp-hydroxybenzoic acid as a specific precursor of coenzyme Q.9 ** Brinkman precoated silica gel G tl plates (20 X 20 cm) were marked into halves, and each half marked into 4 individual tl segments (5 X 10 cm).Four samples could be chromatographed a t one time, and the coenzyme Q isolated from a min quantity of silica gel. The these areas were fringes of the more highly labeled coenzymes Qlo and Q8, resp. I n a typical procedure the coenzymes Qo and Q, areas, cut from the original paper chromatogram, were eluted with EtzO; and each residue, after evapn of Eh0, was applied to separate strips of silicone-impregnated paper. Reversed-phase paper chromatog was performed, as above, and strips corresponding to the areas of reference coenzyme QS and &, , as well aa narrow strips immediately above and below each area, were cut from the paper while still damp with solvent. Coenzymes Q were eluted from each strip with Et20 and the radioactivity in each residue, after evapn of EhO, was measured by liquid scintillation counting.Acknowledgments.-We wish to thank Mr.

No abstract

elude that reaction occurs between the C9 position in the excited dye molecule and oxygen-containing species in the immediate solvation sphere, the chemical nature of the latter being strongly predetermined by pH.The minima for Rhodamine 6G then are construed to represent the transition between acidic and basic solvation spheres, either of which favors hydrol formation. That hydrol may be formed in either acidic or basic media has been confirmed previously.11