Global amphibian declines are one of the biggest challenges currently facing the conservation community, and captive breeding is one way to address this crisis. Using information from the International Species Information System zoo network, we examined trends in global zoo amphibian holdings across species, zoo region, and species geographical region of origin from 1994 to 2014. These trends were compared before and after the 2004 Global Amphibian Assessment to assess whether any changes occurred and whether zoo amphibian conservation effort had increased. The numbers of globally threatened species (GTS) and their proportional representation in global zoo holdings increased and this rate of increase was significantly greater after 2004. North American, European, and Oceanian GTS were best represented in zoos globally, and proportions of Oceanian GTS held increased the most since 2004. South American and Asian GTS had the lowest proportional representation in zoos. At a regional zoo level, European zoos held the lowest proportions of GTS, and this proportion did not increase after 2004. Since 1994, the number of species held in viable populations has increased, and these species are distributed among more institutions. However, as of 2014, zoos held 6.2% of globally threatened amphibians, a much smaller figure than for other vertebrate groups and one that falls considerably short of the number of species for which ex situ management may be desirable. Although the increased effort zoos have put into amphibian conservation over the past 20 years is encouraging, more focus is needed on ex situ conservation priority species. This includes building expertise and capacity in countries that hold them and tracking existing conservation efforts if the evidence-based approach to amphibian conservation planning at a global level is to be further developed.

Scientific studies of human-animal interactions (HAIs) and how these develop into human-animal relationships (HARs) now represent some of the most significant contributions to animal welfare science. However, due to the current definition of HAR, studies have only been able to measure HAIs and infer its impact on HARs and animal welfare. Here, we redefine HARs as a series of repeated HAIs between two individuals known to each other, the nature of which is influenced by their historical HAIs and where consideration to the content, quality and the pattern of the interactions is also vital. With a new definition, it is now feasible to empirically measure HARs, however, first, it is important to evaluate current methods utilised in animal industries to allow standardisation across HAR research in zoos. Here, we review the current methods that have been used to assess HAIs in animals and determine their overall suitability for measuring HARs and their use in a zoo environment. Literature searches were conducted using the search terms 'human-animal' AND 'interaction', 'human-animal' AND 'relationship', 'human-animal' AND 'bond'. Subsequently, 'zoo', 'companion', 'agriculture', 'laboratory' and 'wild' were added to each combination yielding five potential methods to evaluate. These methods were assessed according to a panel of indicators including reliability, robustness, practical application and feasibility for use in a zoo environment. Results indicated that the methods utilising 'latency', 'qualitative behaviour assessment' and the 'voluntary approach test' were potentially viable to assess HARs in a zoo environment and could subsequently contribute to the assessment of welfare implications of these HARs for the animals involved. These methods now require empirical testing and comparisons within a zoo environment.

Human-Animal Relationships (HAR) in zoos develop from repeated interactions between animals and their caretakers. HAR have been shown to affect health and welfare in farm animals, but limited zoo-based studies exist. This study investigates the association between the qualitative behaviour assessment (QBA) of emotional expression in giraffes and keeper action score in four types of keeper-animal interaction (KAI). Three giraffes generating 38 clips. QBA, using a free-choice profiling methodology, was applied instructing 18 observers to assess giraffe expressions shown in these clips. QBA scores were analysed using Generalized Procrustes Analysis. Keeper actions during each KAI event were rated by an independent marker, resulting in cumulative scores for keeper action quality. The association between QBA and the keeper action was analyzed using Spearman’s rank correlations. Two main QBA dimensions were identified explaining 59% of the variation between clips. There were significant effects of giraffe and KAI type on QBA dimension 2 (inquisitive/impatient—calm/distracted), and significant positive associations between keeper action quality rating and QBA dimensions 1 and 2, indicating that positive keeper actions resulted in calm and confident giraffes with a willingness to interact. This is the first successful application of QBA for empirically addressing HARs in zoos, however given the small sample size of giraffes in this study, it can be regarded as a pilot study only, and further research is needed to validate the use of QBA in this context.