ABSTRACT. Within a project on Stone Age sites of NE Germany, 26 burials from the Ostorf cemetery and some further Neolithic sites have been analyzed by more than 40 accelerator mass spectrometry (AMS) dates. We here present the results of stable isotope and radiocarbon measurements together with reference 14

In European and many African, Middle Eastern and Southern Asian populations lactase persistence (LP) is the most strongly selected monogenic trait to have evolved over the last 10,000 years 1 . While LP selection and prehistoric milk consumption must be linked, considerable uncertainty remains concerning their spatiotemporal configuration and specific interactions 2,3 . We provide detailed distributions of milk exploitation across Europe over the last 9k years using c. 7,000 pottery fat residues from >550 archaeological sites. European milk use was widespread from the Neolithic period onwards but varied spatially and temporally in intensity. Surprisingly, comparison of model likelihoods indicates that LP selection varying with levels of prehistoric milk exploitation provides no better explanation of LP allele frequency trajectories than uniform selection since the Neolithic. In the UK Biobank 4,5 cohort of ~500K contemporary Europeans, LP genotype was only weakly associated with milk consumption and did not show consistent associations with improved fitness or health indicators. This suggests other hypotheses on the beneficial effects of LP should be considered for its rapid frequency increase. We propose that lactase non-persistent individuals consumed milk when it became available, but that under particular conditions and microbiological milieux this was disadvantageous, driving LP selection in prehistoric Europe. Comparison of model likelihoods indicates that population fluctuations, settlement density and wild animal exploitationproxies for these driversprovide better explanations of LP selection than the extent of milk exploitation. These findings offer new perspectives on prehistoric milk exploitation and LP evolution.

Modern humans have populated Europe for more than 45,000 years1,2. Our knowledge of the genetic relatedness and structure of ancient hunter-gatherers is however limited, owing to the scarceness and poor molecular preservation of human remains from that period3. Here we analyse 356 ancient hunter-gatherer genomes, including new genomic data for 116 individuals from 14 countries in western and central Eurasia, spanning between 35,000 and 5,000 years ago. We identify a genetic ancestry profile in individuals associated with Upper Palaeolithic Gravettian assemblages from western Europe that is distinct from contemporaneous groups related to this archaeological culture in central and southern Europe4, but resembles that of preceding individuals associated with the Aurignacian culture. This ancestry profile survived during the Last Glacial Maximum (25,000 to 19,000 years ago) in human populations from southwestern Europe associated with the Solutrean culture, and with the following Magdalenian culture that re-expanded northeastward after the Last Glacial Maximum. Conversely, we reveal a genetic turnover in southern Europe suggesting a local replacement of human groups around the time of the Last Glacial Maximum, accompanied by a north-to-south dispersal of populations associated with the Epigravettian culture. From at least 14,000 years ago, an ancestry related to this culture spread from the south across the rest of Europe, largely replacing the Magdalenian-associated gene pool. After a period of limited admixture that spanned the beginning of the Mesolithic, we find genetic interactions between western and eastern European hunter-gatherers, who were also characterized by marked differences in phenotypically relevant variants.

Sea-level change has influenced human populations globally since prehistoric times. Even in early phases of cultural development, human populations were faced with marine regression and transgression associated with the glacial -interglacial climatic cycle, amplified by glacio-isostatic adjustments in some regions. Global marine regression during the last glaciation changed the palaeogeography of the continental shelf, converting former marine environments to attractive terrestrial habitats for prehistoric human occupation, and adding an extensive new increment of land, in the case of Europe amounting to an additional 40% of the existing land area, and on a global scale to some 20 million km 2 of additional territory that is now submerged. These areas of the shelf were used as hunting and gathering areas, and as pathways of dispersal between regions and between continents, until they were resubmerged by the post-glacial marine transgression. They also most probably witnessed the earliest developments in seafaring, marine exploitation and permanent settlements. Based on modern marine research technologies and the integration of large databases, proxy data are now becoming available for the reconstruction of these submerged Quaternary landscapes. Concerted efforts are also now being devoted to the search for prehistoric archaeological sites and artefacts on the seabed, often in collaboration with marine scientists. This search has been stimulated by the increasing amount of material that has demonstrably survived inundation, often with excellent preservation of organic remains, by closer collaboration with offshore industry, and by the growing realization of the importance of these submerged data for understanding human prehistoric developments during periods of rapidly changing climate and environment. Moreover, these new research trends are not simply being driven by an archaeological need for scientific and technological input from other disciplines, but by collaborations involving genuine mutual benefit, in which all partners have something to gain. Archaeological problems often pose new questions about geological change, stimulating new techniques of observation, new technologies and new investigations, which in their turn can offer new data, often at higher resolution and with better dates, in relation to geological and environmental issues such as sea-level change and palaeoclimatic variability on the continental shelf.The expansion of early human populations to occupy new territory and new continents is one of the great narratives of human evolution. It is currently a theme of wide interest and topicality, and has received significant impetus and publicity from the new science of palaeogenetics. Together with ongoing discoveries of new fossil and archaeological material, and new studies of palaeoclimate and palaeoenvironment, the field itself is rapidly expanding, leading to new discoveries and new controversies (Grine et al. 2009;Gamble 2013; Smith & Ahern 2013). All the current indications are that the ...

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