In the present work we have estimated the effect of acute heat stress (1 hour to 41±0,5°C) and the different period of recovery at room temperature after the heat stress (0, 30 minutes, 1, 6, 12, 24, 48 and 96 hours) over the hepatic carbohydrate-related enzymes and substrates of rats.The obtained results can be summarized in three phases: up to 6 th hour, from 6 th to 24 th hour and form 24 th up to 96 th hour of recovery after the heat stress. Acute heat stress (up to 1hour of recovery) caused intensive glycogenolysis, associated with moderate, but non-significant endogenous production of glucose. The period of 6 th to 24 th hour of recovery after exposure to acute heat stress, is characterized with intensive rebound of glycogen stores, with decreased glucose production, and finally, from the 48 th up to 96 th hour of recovery, process of glycogenolysis is still evident, even though less intensive than the first hours after the heat stress.Overall, we can conclude that acute heat stress and the period of recovery after the heat stress are followed with dynamic changes of the liver carbohydrate-relates enzymes and substrates in a course of elevated glycogenolysis, but decreased glycolysis and gluconeogenesis.