A pilot-scale combined system composed of anaerobic digenstion, membrane bioreactor (MBR) and Electro-Catalyzed oxidation processes has been set up to treat the leachate from municipal solid waste (MSW) transfer station. System performance was evaluated by ammonia and chemical oxidation demand (COD) removal . Results showed that 97% of ammonia was removed by MBR, and the elimination of COD was mainly completed in anaerobic process and MBR with a total removal of 99%. In addition, the analysis of microbial communities in the MBR, carried out by clone library and denaturing gradient gel electrophoresis (DGGE) of the 16S rRNA genes, indicated a highly diverse bacterial community and the enrichment of N-cycling bacteria.

Sulfite pretreatment was explored in enzymatic hydrolysis of bagasse for the first time, and was compared with dilute acid pretreatment. The results showed that enzymatic hydrolysis yield of bagasse after sulfite treatment was lower than that of the bagasse pretreated with dilute acid. Meanwhile, complexity of sulfite pretreatment and its high cost made it infeasible for industrialized production. Results also showed positive correlation of bagasse pretreatment weight loss to enzymatic hydrolysis yield in dilute acid pretreatment processes, which made substrate weight loss a plausible parameter in pretreatment evaluation.