In this article, the layered deposition method is adopted to prepare the collagen/hydroxyapatite (COL/HA) composite coating. The morphology and elements of the COL/HA composite coating are observed using a scanning electron microscope (SEM) with an energy dispersive spectrometer (EDS), the optical density (O.D.) values are obtained by MTT assay to assess the cellular viabilityof composite coating. The experimental results showed that the addition of collagen not only improve the bonding strength of composite coating and porous titanium substrate, but also combine the osteoconduction of inorganic coating HA and the osteoinduction of organic coating COL, effectively enhance the cellular adhesion, proliferation and differentiation. The cellular viability cultured in COL/HA composite coating is much higher than the pure HA modified coating.

Porous Ti6Al4V alloys with anisotropic structure for biomedical application was fabricated by diffusion bonding of titanium alloy meshes. Compressive mechanical compatibility of the alloys is investigated as human bone implants. It is concluded that the fabrication processing for porous Ti6Al4V alloys has better control of the porosity. The pore structure of porous titanium is anisotropic, with elongated and square pores in the out-of-plane and in-plane direction, respectively, which is suited for bone ingrowth. The compressive Young’s modulus and yield stress of porous Ti6Al4V alloy compressed in the out-of-plane direction are 12.2 GPa and 171.4 MPa, respectively, which is compatible with those for the cortical bones.