Polyherbal additives represent an alternative to enhance the productivity in dairy cattle. Some herbal mixtures with conjugates of choline and methionine have improved milk yield in dairy cattle. An experiment was conducted to evaluate two herbal formulas as sources of choline and methionine on dairy cows production and metabolites change. Thirty-two Holstein cows (Body Condition Score 3.01 ± 0.16) fed a basal diet, were randomly assigned to one of four treatments: Control diet, Biocholine (BIO) supplementation (15 grams/cow/day), Optimethionine (OP) supplementation (9 grams/cow/day), and BIO x OP supplementation, with a duration of 60 days. Optimethionine treatments decrease milk production (P ≤ 0.05), but fat corrected production was higher (P ≤ 0.05) when cows were supplemented with BIO. The chemical composition of the milk did not show differences between treatments. Plasma urea and cholesterol were not different between treatments, glucose shown a decrease (P ≤ 0.05) whit supplementation. Aspartate transferase activity decrease (P ≤ 0.05) with BIO supplementation. Results indicate that the herbal BIO and OP present some productive and metabolic changes.