Aims: This study aims to investigate the inhibitory susceptibility of extract Sidr leaf (Ziziphus spina-christi) aqueous, silver nanoparticles Ag NPs and Sidr nanoparticle "Nano-sidr" (Ziziphus spina-christi) against local isolates of Staphylococcus.SPP, and their inhibition effect on biofilm formation. Study Design: Initially diagnosed by The use of Mannitol salt agar medium, then 26 isolates were selected from them depending on the resistance to methicilln and Vancomycin that were conducted. Place and Duration of Study: The samples were collected from AL-Najaf AL-Ashraf and Baghdad hospitals. Methodology: These isolates were subjected to a VITEK-2 compact system "ID, AST", to ascertain the genus and type of Staphylococcus bacteria., morphological and biochemical tests were conducted on them to confirm them. Results: The result of the diagnosis showed 10 isolates belonging to Staphylococcus aureus, and a number of them were resistant to as MRSA-VRSA, and 7 were Staphylococcus haemolyticus and they were all resistant and known as MRSA-VRSA, and two isolates of Staphylococcus sciuri bacteria, one of them was resistant to VRSA and the other to MRSA-VRSA , two isolates of Staphylococcus warneri were both resistant to MRSA, two isolates of Staphylococcus lugdunensis were resistant to antibiotics, one of them was VRSA and the other was MRSA-VRSA, and one isolate of Staphylococcus lentus was also resistant to antibiotics MRSA-VRSA, and one isolate of Staphylococcus warneri was resistant to MRSA-VRSA, and one isolate of Staphylococcus lugdunensis was resistant to antibiotics. Conclusion: Isolation of Staphylococcus vitulinus. 11 different isolates were selected from them according to their resistance to antibiotics, and after selecting the most efficient one by examining the inhibitory activity by diffusion method. The results showed the ability of both types of nanoparticles. Plant extracts prevent the formation of biofilms