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In view of the main problems existing in the training and practical teaching of applied and innovative talents in China, it is studied in the paper that the construction of practical teaching system for applied undergraduate talents. It is explored the reform of practical teaching contents and methods, and the optimization of teaching methods. This paper expounds how to build up a team of experimental teaching instructors to ensure the quality and level of experimental teaching. It is emphasized to improve the management system of experimental teaching system and the monitoring and evaluation mechanism of experimental teaching quality.