The aim of this study was to quantify the movement patterns of various playing positions during professional rugby union match-play, such that the relative importance of aerobic and anaerobic energy pathways to performance could be estimated. Video analysis was conducted of individual players (n=29) from the Otago Highlanders during six "Super 12" representative fixtures. Each movement was coded as one of six speeds of locomotion (standing still, walking, jogging, cruising, sprinting, and utility), three states of non-running intensive exertion (rucking/mauling, tackling, and scrummaging), and three discrete activities (kicking, jumping, passing). The results indicated significant demands on all energy systems in all playing positions, yet implied a greater reliance on anaerobic glycolytic metabolism in forwards, due primarily to their regular involvement in non-running intense activities such as rucking, mauling, scrummaging, and tackling. Positional group comparisons indicated that while the greatest differences existed between forwards and backs, each positional group had its own unique demands. Front row forwards were mostly involved in activities involving gaining/retaining possession, back row forwards tended to play more of a pseudo back-line role, performing less rucking/mauling than front row forwards, yet being more involved in aspects of broken play such as sprinting and tackling. While outside backs tended to specialize in the running aspects of play, inside backs tended to show greater involvement in confrontational aspects of play such as rucking/mauling and tackling. These results suggest that rugby training and fitness testing should be tailored specifically to positional groups rather than simply differentiating between forwards and backs.

Field experiments were carried out at seven sites in southern Australia from 2002 to 2006 to measure changes in plant frequency, root characteristics and summer activity for a range of grass and herb species or cultivars. Annual rainfall during the experimental period was on average 75 mm lower than the long-term average. Plant frequency differed significantly between species and between sites. Temperate grasses generally had higher frequencies than subtropical grasses, native grasses and herbs. Cocksfoot (Dactylis glomerata cvv. Currie, Porto), tall wheat grass (Thinopyrum ponticum cv. Dundas), winter-active tall fescue (Festuca arundinacea cvv. Fraydo, Resolute MaxP) and phalaris (Phalaris aquatica cvv. Atlas PG, Australian) were the most persistent of the temperate perennial species over the experimental period. The frequency of most cultivars declined from year 2 to year 4 after establishment, but the frequency of kikuyu (Pennisetum clandestinum cv.Whittet) and wallaby grass (Austrodanthonia richardsonii cv. Taranna) increased by over 5% from year 2 to year 3, and cocksfoot (cv. Currie) increased from year 3 to year 4. At two sites where measurements were made, there were significant differences in rooting depth between species. Whittet kikuyu was the deepest among all species with a rooting depth of up to 2 m, followed by phalaris, tall fescue, grazing brome (Bromus stamineus) and tall wheat grass. Root density was affected by plant genotype and soil structure. Root density of the species varied significantly in the subsoil (0.1–1.1 m) and deeper subsoil (1.1–2 m) but not in the topsoil (0–0.1 m). Green-leafiness over summer was generally higher for subtropical grasses, native grasses, herbs and some summer-active temperate grasses, than most temperate grasses with high summer dormancy.

Genetic map construction and identification of quantitative trait loci (QTLs) for blackleg resistance were performed for four mapping populations derived from five different canola source cultivars. Three of the populations were generated from crosses between single genotypes from the blackleg-resistant cultivars Caiman, Camberra and (AV)Sapphire and the blackleg-susceptible cultivar Westar(10). The fourth population was derived from a cross between genotypes from two blackleg resistant varieties (Rainbow and (AV)Sapphire). Different types of DNA-based markers were designed and characterised from a collection of 20,000 EST sequences generated from multiple Brassica species, including a new set of 445 EST-SSR markers of high value to the international community. Multiple molecular genetic marker systems were used to construct linkage maps with locus numbers varying between 219 and 468, and coverage ranging from 1173 to 1800 cM. The proportion of polymorphic markers assigned to map locations varied from 70 to 89% across the four populations. Publicly available simple sequence repeat markers were used to assign linkage groups to reference nomenclature, and a sub-set of mapped markers were also screened on the Tapidor x Ningyou (T x N) reference population to assist this process. QTL analysis was performed based on percentage survival at low and high disease pressure sites. Multiple QTLs were identified across the four mapping populations, accounting for 13-33% of phenotypic variance (V (p)). QTL-linked marker data are suitable for implementation in breeding for disease resistance in Australian canola cultivars. However, the likelihood of shifts in pathogen race structure across different geographical locations may have implications for the long-term durability of such associations.

Abstract. The single largest influence on the survival of lambs in the first few days of life is their birthweight. Fetal growth and birthweight are regulated by genotype of the fetus, maternal genotype, maternal nutrition and the external environment. In this paper we report the extent to which the changes in maternal liveweight during pregnancy and lactation (liveweight profile) of Merino ewes can be used to predict the birthweight and survival of their progeny to weaning. At two sites [Victoria (Vic.)~700 ewes and Western Australia (WA)~300 ewes] in each of 2 years, a similar experiment used adult Merino ewes to explore effects of nutrition from joining to Day 100 of pregnancy and from Day 100 of pregnancy to weaning. The average difference between extreme treatments at Day 100 of pregnancy were 7 kg in ewe liveweight and 0.7 of a condition score (CS) and at lambing 11.9 kg and 1.3 of a CS. This resulted in average birthweights of progeny from different treatments ranging from 4.0 to 5.4 kg and survival to weaning ranging from 68 to 92%. Across the four experiments between 68 and 85% of all lamb deaths to weaning occurred within 48 h of birth. Lambs born to ewes in CS 2 at Day 100 of pregnancy were lighter (P < 0.05) in both years at the Vic. site than those from ewes in CS 3 at Day 100 of pregnancy. Lambs born to the ewes grazing a feed on offer of 800 kg DM/ha during late pregnancy were also lighter than those from other levels of feed on offer between 1100 and 3000 kg DM/ha at the Vic. site in both years and at the WA site in 1 year (P < 0.001). Lambs from the 800 kg DM/ha treatment during late pregnancy at the Vic. site had a lower survival than other treatments, especially in the second year. There were no significant effects of treatments on lamb survival at the WA site; however, the results were in the same direction. The birthweight of individual lambs was significantly related to the liveweight profile of their mothers. Their liveweight at joining, change in liveweight to Day 100 of pregnancy and change in liveweight from Day 100 to lambing all contributed (P < 0.05) to the prediction of the birthweight of their lambs. The responses were consistent across experimental sites and years, lamb birth rank and sex, and confirmed that the effects of poor nutrition up until Day 100 of pregnancy could be completely overcome by improving nutrition during late pregnancy. At the Vic. site, survival to 48 h was most influenced by the birthweight of the lamb and survival was significantly higher in single-than twin-born lambs and female than male lambs after adjusting for differences in birthweight. A higher chill index during the 48 h after birth reduced survival of both single and twin lambs to a similar extent, but reduced survival of male lambs more than female lambs. There were no effects of birthweight or chill index on lamb survival at the WA site where most lambs weighed more than 4 kg at birth and climatic conditions during lambing were less extreme. Overall, these results supported our hypothesis that im...

Abstract. Defining the nature of the relationship between change in liveweight throughout a breeding cycle and ewe wool production and reproduction would be useful for developing management guidelines for Merino ewes. In this paper we tested the hypotheses that (1) feed on offer has variable effects on liveweight profiles of individual ewes; and (2) liveweight profiles of individual ewes can be used to predict their fleece wool production and reproductive performance. At sites in Victoria and Western Australia in 2001 and 2002, pregnant Merino ewes were exposed to 10 nutritional treatments. In each of the four experiments, ewes in average condition score 3 at artificial insemination were fed to achieve either maintenance or loss of a condition score over the first 100 days of pregnancy before grazing one of five levels of feed on offer between Day 100 and lamb weaning. Across all four experiments, the average difference in ewe liveweight between extreme treatments was: 7.0 kg (range 4.7-8.7 kg) at Day 100 of pregnancy; 11.9 kg (range 4.9-17.8 kg) at lambing; and by weaning was 13.9 kg (range 8.8-22.7 kg). Liveweight at joining and liveweight change during pregnancy and lactation of individual Merino ewes were significantly related to their clean fleece weight, fibre diameter and staple length and thus the second hypothesis was supported. Heavier ewes at joining produced more wool that was longer and broader and this effect was consistent across both sites and years. A 10-kg loss in ewe liveweight between joining and mid pregnancy, mid pregnancy and lambing or during lactation reduced clean fleece weight by 0.4-0.7 kg and fibre diameter by 0.5-1.4 um. At the Victorian site, where ewes were shorn in summer, a loss of 10 kg in liveweight between joining and Day 100 of pregnancy reduced staple strength by 5 N/ktex. As expected the influence of food on offer on changes in ewe liveweight was different between years and sites and between late pregnancy and lactation due to a complex group of pasture and animal factors. Therefore, managing changes in ewe liveweight itself rather than feed on offer will achieve more predictable outcomes. A higher liveweight at joining resulted in a predictable improvement in ewe reproductive rate and liveweight at joining was more important than the liveweight profile leading up to joining. This paper has shown that it is possible to predict the differences in wool production and reproductive rate of flocks of Merino ewes if ewe liveweight records at key times are known.