This study was aimed to investigate the extent of pollution in Poultry slaughterhouse with Salmonella spp and their effect on public health .(142) samplescollected from (8) poultry slaughter houses in Baghdad were examined . thesesamples included( live chicken cages ,live chicken transporting cars,slaughtering knives ,workers hands, transport belt , chiller , chick beforepackaging stage) .All samples were cultured on special media until the growthappeared then Biochemical tests were done for diagnosis of Salmonella spp.The results of this study shown the diagnosis of (14) isolates of Salmonella spp,all of them were motile except (3) isolates, These isolates were recovered fromthe following sites:1. (2) isolates from live chicken transporting cars.2. (1) isolate from live chicken cages.3. (3) isolates from workers hands.4. (1) isolate from transport belt.5. (1) isolate from slaughtering knives.6. (3) isolates from chiller.7. (2) isolates from stunning.8. (2) isolates from chickens beforepackaging.

Summary 250 chickens which had been suspected to have died because of salmonellosis were submitted for bacteriological examination. 30 were positive and 5 serotypes were isolated and identified. These are: Sal. baghdad (new serotype); Sal. senftenberg; Sal. bareilly; Sal. typhi‐murium; Sal. typhi‐murium Copenhagen and eleven untyped Salmonella serotypes. Zusammenfasung Das Vorkommen von Salmonellen bei Küken im Irak 250 Küken, die vermutlich an Salmonellose verendet waren, wurden bakteriologisch untersucht. 30 waren positiv, und fünf Serotypen konnten isoliert und identifiziert werden. Es handelte sich um: Sal. baghdad (neuer Serotyp); Sal. senftenberg; Sal. bareilly; Sal. typhi‐murium; Sal. typhi‐murium Kopenhagen und elf nicht typisierte Salmonella Serotypen.

The influence of heat and cold on the L.monocytogenes in the milkIn order to know the thermal resistance of L.monocytogenesin thecontaminated milk, 40gram of powder milk was dissolved in 400mlof heated water.The milk solution was sealed in 40 test tubes 10 mlin each one.these test tubes were divided into 6 groups ,10test tubesin each the 1st,2nd group &5 test tubes in each the 3rd ,4th ,5th ,6thgroups,then all the test tubes were inoculated withL.monocytogenes &incubation for 24hrs at 37c.the samples of the1st &2nd group were dipped in awater bath at tempreture 63c for30minute &72c for 1-5.5 minute respectively .also the samples of3rd &4th groups were dipped in awater bath at 72c for 6minute. Testtubes of 1st ,3rd ,5th groups were kept in 4c for variable times whilesamples of 4th ,6th group were exposured to - 18c for 12days. Theresults showed no bacterial isolates immediately after heattreatment in all the milk samples of 1st .3rd &4th groups but someisolates were seen after enrichment at 4c&- 18c L.monocytogeneswas isolate from all samples of 2nd ,5th &6th groups. The resultssuugest that cold enrichment play role in repair the thermallyinjured cells of L.monocytogenes&these organisms survivevethermal treatment.

 The isolation and identification of the bacterium  L.monocytogenes among abortion in pregnant women were investigated in this research. One hundred and fifty samples including placental tissue and maternal blood were randomly collected from cases of abortion from AL-Habibia Teaching Hospital and AL-Yarmuk Teaching Hospital. These samples were taken from patients in 16 - 45 years of age. Listeria monocytogenes was isolated from three cases only, two of them  from AL-Habibia Teaching Hospital ,while the other one from AL-yarmuk Teaching Hospital. Their gestational age were 22 , 24 , and 28 weeks. Bacterial isolation was done by using routin and specific culture media and two methods for enrichment were preformed.  The biological and biochemical properties of the isolates as well as the pathogenicity of L.monocytogenes in mice were described in the original paper. Infected mice were died after 2 - 5 days post inoculation. The histopathological changes were acute suppurative inflammation in the begining « later on chronic granulomatous reaction were developed , within internal organs

In order to study some aspects of pathogenesis of Rhodococcus equi, 60 white Swiss mice were used. They were randomly divided into 2 groups. The 1s' group (40 animals) was inoculated subcutaneously with a dose of 4x10° CFU .of R.equi while the 2nd group was kept as a control. Four animals from the 1st group and 2 animals from the 2nd group were sacrified at day, 1,2,4,8,12,16,20,24,28 and 30 postinoculation (P.I) Six animals of 1stgroup were died during the first and second days P.I. Microscopical examination showed acute to subacute suppurative inflammation in the liver, lung and spleen at I to 4 days PI. While pyogranulomatous lesions and epithelioid granuloma were seen in liver, lungs and spleen at 4 to 24 days PI R.equi was isolated liver, lungs, spleen, kidney and brain in the first day PI. The bacteria persisted in the spleen from and brain up to 20th day, in the liver up to 24" day while in the lungs, and kidney bacterial isolation continuous till 28th day PI.