Simple sequence repeat (SSRs) of DNA are subject to high rates of mutation and are important mediators of adaptation in Haemophilus influenzae. Previous studies of the Rd KW20 genome identified the primacy of tetranucleotide SSRs in mediating phase variation (the rapid reversible switching of gene expression) of surface exposed structures such as lipopolysaccharide. The recent sequencing of the genomes of multiple strains of H. influenzae allowed the comparison of the SSRs (repeat units of one to nine nucleotides in length) in detail across four complete H. influenzae genomes and then comparison with a further 12 genomes when they became available. The SSR loci were broadly classified into three groups: (1) those that did not vary; (2) those for which some variation between strains was observed but this could not be linked to variation of gene expression; and (3) those that both varied and were located in regions consistent with mediating phase variable gene expression. Comparative analysis of 988 SSR associated loci confirmed that tetranucleotide repeats were the major mediators of phase variation and extended the repertoire of known tetranucleotide SSR loci by identifying ten previously uncharacterised tetranucleotide SSR loci with the potential to mediate phase variation which were unequally distributed across the H. influenzae pan-genome. Further, analysis of non-tetranucleotide SSR in the 16 strains revealed a number of mononucleotide, dinucleotide, pentanucleotide, heptanucleotide, and octanucleotide SSRs which were consistent with these tracts mediating phase variation. This study substantiates previous findings as to the important role that tetranucleotide SSRs play in H. influenzae biology. Two Brazilian isolates showed the most variation in their complement of SSRs suggesting the possibility of geographic and phenotypic influences on SSR distribution.

BackgroundSialic acid has been shown to be a major virulence determinant in the pathogenesis of otitis media caused by the bacterium Haemophilus influenzae. This study aimed to characterise the expression of genes required for the metabolism of sialic acid and to investigate the role of these genes in virulence.ResultsUsing qRT-PCR, we observed decreased transcriptional activity of genes within a cluster that are required for uptake and catabolism of 5-acetyl neuraminic acid (Neu5Ac), when bacteria were cultured in the presence of the sugar. We show that these uptake and catabolic genes, including a sialic acid regulatory gene (siaR), are highly conserved in the H. influenzae natural population. Mutant strains were constructed for seven of the nine genes and their influence upon LPS sialylation and resistance of the bacteria to the killing effect of normal human serum were assessed. Mutations in the Neu5Ac uptake (TRAP transporter) genes decreased virulence in the chinchilla model of otitis media, but the attenuation was strain dependent. In contrast, mutations in catabolism genes and genes regulating sialic acid metabolism (siaR and crp) did not attenuate virulence.ConclusionThe commensal and pathogenic behaviour of H. influenzae involves LPS sialylation that can be influenced by a complex regulatory interplay of sialometabolism genes.

Aim :Methodology : Results :Interpretation :The present study was conducted to investigate the genotoxic effect of UV-B radiation on morphological, biochemical and cytological parameters of Cuminum cyminum.The germinated roots of cumin were exposed to UV-B radiation at different intervals viz., 20 min 40 min and 60 min, respectively, and some treated seeds were sown on pots for morphological and biochemical observation.Chromosomal studies divulged that UV-B radiation has substantial impact on Active Mitotic Index (AMI %). On increasing UV-B radiation dose, the chromosomal aberration rate elevated, thus AMI % moderately decreased and Total Abnormality percentage (TAB %) gradually increased. Different types of chromosomal abnormalities were a s c e r t a i n e d , a m o n g w h i c h scattering was more prominent. The morphological observation showed that survival percentage and plant height decreased at elevated dose of UV-B. Biochemical results indicated that chlorophyll a, chlorophyll b and carotenoid contents decreased as compared to control, but proline c o n t e n t s h o w e d s i g n i f i c a n t increment at higher doses of UV-B.The observation elucidates that UV-B causes chromosomal aberrations during cell division and acts as a potent genotoxic agents for roots. Thus, it can be concluded from the above experiment that UV-B rays promote plant growth at low doses but at the higher doses, it subsequently hinders plant growth by damaging important constituents of plant cell.