Biofertilizers have been used as sources to improve plant nutrients in sustainable agriculture. Biofertilizers are low cost, renewable sources of plant nutrients which supplement chemical fertilizers. Mycorrhizal fungi are a major component of agricultural natural resources and members of kingdom fungi. The term 'mycorrhiza' is derived from Greek word which means 'fungus root'. Earthen pot experiments were carried out to study the inoculation effect of Arbuscular Mycorrhizal (AM) fungi (Glomus mossease), Azospirillium brasilense and PSB on plant height, dry weight of root and shoot, per cent root colonization, spore number, P and N uptake. Experimental pots were filled with 4 kegs of sterilized soil and maintained in green house at 25-30 °C temperature. Single inoculation of AM fungi and combined inoculation of AM fungi with Azospirillium brasilense or PSB was found to be moderately increased in all the growth parameters. However triple inoculation of AM fungi, Azospirillium brasilense and PSB was found to have highest growth parameters.

The present research was carried out to evaluate response of Bell peppers (Capsicum annuum L.) to plant density and nitrogen fertilizer under field conditions. Plant density at four levels (20×50 cm, 30×50 cm, 20×100 cm and 30×100 cm) and nitrogen treatments at four levels (0, 50, 100 and 150 kg·N·ha -1 ) were applied. Plant height, lateral stem number, leaf chlorophyll content, yield, and were assessed at immature and mature stages. The results showed that vegetative growth characteristics (plant height, lateral stem number and leaf dry matter) and reproductive factors (fruit volume and fruit weight) decreased with increasing plant density, but total yield (kg·ha -1 ) increased with increasing plant density. The highest and lowest total yields were obtained by plant density 20×50 cm and 30×100 cm respectively. Nitrogen fertilizer was significantly affected on plant height, lateral stem number and leaf chlorophyll content. It was observed that fertilization with 150 kg·N·ha -1 resulted to the highest fruit volume and plant yield. There were significant differences between fruit volume and fruit weight by interaction between plant density and nitrogen treatments.

A study was conducted to determine the Production of cellulase and pectinase enzyme by using Plant growth promoting rhizobacteria like Pseudomonas fluorescence and Bacillus subtilis. These to micro organism are isolated by serial dilution method. One gram of soil sample was diluted in to 10 ml of sterile distilled water and 1 ml of sample solution was serially diluted in 9ml of sterile water up to 10 dilution. Each sample from dilution 10-5 and 10-6 were taken and streaked in to KB and NA medium and incubate at 24 hrs. After 24 hrs Pseudomonas fluorescence and Bacillus subtilis was observed in the medium of KB and NA medium. Both the culture was sub cultured and maintain in the same for the further work. CMCase medium was prepared and sterilized by autoclave for 121 °C for 15 minutes after sterilization these medium contain petriplate was streaked by bacteria and incubates for 48h after incubation period a clear halo zone was produced by these bacteria among these bacteria Pseudomonas fluorescence are able to produce high amount of cellulose compare to Bacillussubtilis. Pectin agar medium was prepared and sterilized by autoclave for 121 °C for 15 minutes after sterilization these medium contain petriplate was streaked by bacteria incubates for 48h after incubation period a clear halo zone was produced by these bacteria, among these bacteria Pseudomonas fluorescence are able to produce high amount of Pectinase compare to Bacillus subtilis. Plant growth promoting rhizobacteria (PGPR) are beneficial bacteria that colonize plant roots and enhance plant growth by a wide variety of mechanisms.

A study was conducted to determine the Production of cellulase and pectinase enzyme by using Plant growth promoting rhizobacteria like Pseudomonas fluorescence and Bacillus subtilis. These to micro organism are isolated by serial dilution method. One gram of soil sample was diluted in to 10 ml of sterile distilled water and 1 ml of sample solution was serially diluted in 9ml of sterile water up to 10 dilution. Each sample from dilution 10 -5 and 10 -6 were taken and streaked in to KB and NA medium and incubate at 24 hrs. After 24 hrs Pseudomonas fluorescence and Bacillus subtilis was observed in the medium of KB and NA medium. Both the culture was sub cultured and maintain in the same for the further work. CMCase medium was prepared and sterilized by autoclave for 121 ºC for 15 minutes after sterilization these medium contain petriplate was streaked by bacteria and incubates for 48h after incubation period a clear halo zone was produced by these bacteria among these bacteria Pseudomonas fluorescence are able to produce high amount of cellulose compare to Bacillus subtilis. Pectin agar medium was prepared and sterilized by autoclave for 121 ºC for 15 minutes after sterilization these medium contain petriplate was streaked by bacteria incubates for 48h after incubation period a clear halo zone was produced by these bacteria, among these bacteria Pseudomonas fluorescence are able to produce high amount of Pectinase compare to Bacillus subtilis. Plant growth promoting rhizobacteria (PGPR) are beneficial bacteria that colonize plant roots and enhance plant growth by a wide variety of mechanisms.

The present study reveals the pharmacognostic studies root of Stachytarpheta jamaicensis (L) Vahl. The objectives of the present work comprises of collection, identification, macroscopical, Ph of aqueous solution, soluble ash, alcohol soluble extractive, flouresence analysis of root. The root section was taken and microscopical studies were carried out, the root shows derived from the cork, the periderm tissue is formed, which replaces the epidermis in the outer layer. The next tissue identified inwards was the cortex. Sclerenchyma was seen as a ring in the roots, while as a group, formed from a group of cells, in the mature ones. In the middle, xylem tissue occupies a large area, just below the phloem tissue.