[1] We examine magnetic field data from 10 apoapsis passes of the Cassini spacecraft during 2006 when the spacecraft explored the midnight and dawn sectors of Saturn's magnetotail to down-tail distances of $65 R S (Saturn radius, R S , is 60,268 km). Oscillations in the radial component of the field near the $11 hour planetary period associated with north-south motions of the current sheet are ubiquitous in these data. Here, we examine and model the phase of these oscillations throughout the interval, taking account of both local time and radial propagation effects, and show that the oscillations exhibit dual periodicities. Those observed at distances exceeding $3 R S north of the modeled average center of the current sheet are found to oscillate near the modulation period of the northern Saturn kilometric radiation (SKR) emissions, while those observed south of this location oscillate near the modulation period of the southern SKR emissions. The phasing in both cases is consistent with the sense of the associated rotating quasi-uniform perturbation fields within the quasi-dipolar "core" region. We determine the structure of the current sheet as a function of the modeled phases, the results implying that the form of the modulation varies significantly over the beat cycle of the two oscillations. When the two field oscillations are in phase, the current sheet oscillates north-south with a peak-to-peak amplitude of $3 R S . When they are in antiphase, however, the thickness of the current sheet is also strongly modulated during the oscillation by factors of $2.

High microvessel density, an indirect measure of angiogenesis, has been shown to correlate with increased tumour size, lymph node involvement and poor prognosis in non-small-cell lung cancer (NSCLC). Tumour cell vascular endothelial growth factor (VEGF) and platelet-derived endothelial cell growth factor (PD-ECGF) expression correlate with angiogenesis and a poor outcome in this disease. In a retrospective study VEGF and PD-ECGF expression and microvessel density were evaluated immunohistochemically in surgically resected specimens (T1–3, N0–2) from 223 patients with operable NSCLC using the VG1, P-GF.44C and JC70 monoclonal antibodies respectively. High VEGF immunoreactivity was seen in 104 (46.6%) and PD-ECGF in 72 (32.3%) cases and both were associated with high vascular grade tumours ( P = 0.009 and P = 0.05 respectively). Linear regression analysis revealed a weak positive correlation between VEGF and PD-ECGF expression in cancer cells ( r = 0.21; P = 0.002). Co-expression of VEGF and PD-ECGF was not associated with a higher microvessel density than VEGF or PD-ECGF only expressing tumours. Furthermore a proportion of high vascular grade tumours expressed neither growth factor. Univariate analysis revealed tumour size, nodal status, microvessel density and VEGF and PD-ECGF expression as significant prognostic factors. Tumour size ( P < 0.02) and microvessel density ( P < 0.04) remained significant on multivariate analysis. In conclusion, VEGF and PD-ECGF are important angiogenic growth factors and have prognostic significance in NSCLC. Furthermore the study underlines the prognostic significance of microvessel density in operable NSCLC. © 2000 Cancer Research Campaign

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Hypoxia-inducible factor (HIF)-1␣ is the regulatory subunit of HIF-1 that is stabilized under hypoxic conditions. Under different circumstances, HIF-1␣ may promote both tumorigenesis and apoptosis. There is conflicting data on the importance of HIF-1␣ as a prognostic factor. This study evaluated HIF-1␣ expression in 172 consecutive patients with stage I-IIIA non small cell lung cancer (NSCLC) using standard immunohistochemical techniques. The extent of HIF-1␣ nuclear immunostaining was determined using light microscopy and the results were analyzed using the median (5%) as a low cut-point and 60% as a high positive cut-point.

Summary Angiogenesis is essential for tumour growth beyond 1 to 2 mm in diameter. The clinical relevance of angiogenesis, as assessed by microvessel density (MVD), is unclear in malignant mesothelioma (MM). Immunohistochemistry was performed on 104 archival, paraffinembedded, surgically resected MM samples with an anti-CD34 monoclonal antibody, using the Streptavidin-biotin complex immunoperoxidase technique. 93 cases were suitable for microvessel quantification. MVD was obtained from 3 intratumoural hotspots, using a Chalkley eyepiece graticule at × 250 power. MVD was correlated with survival by Kaplan-Meier and log-rank analysis. A stepwise, multivariate Cox model was used to compare MVD with known prognostic factors and the EORTC and CALGB prognostic scoring systems. Overall median survival from the date of diagnosis was 5.0 months. Increasing MVD was a poor prognostic factor in univariate analysis (P = 0.02). Independent indicators of poor prognosis in multivariate analysis were non-epithelial cell type (P = 0.002), performance status > 0 (P = 0.003) and increasing MVD (P = 0.01). In multivariate Cox analysis, MVD contributed independently to the EORTC (P = 0.006), but not to the CALGB (P = 0.1), prognostic groups. Angiogenesis, as assessed by MVD, is a poor prognostic factor in MM, independent of other clinicopathological variables and the EORTC prognostic scoring system. Further work is required to assess the prognostic importance of angiogenic regulatory factors in this disease.