The objective of this review article is to review literature on fatigue analysis and biomaterials studies in Endodontics. The nitinol and gutta-percha are widely used biomaterials in the Endodontic fields. The nitinol is used to manufacture the Endodontic files which are used for preparation of the root canal. The preparation of the root canal consist of removal of dead tissue, substrates and debris from the decayed root canal. During the preparation of root canal shaping of the root canal is done to develop the perfectly prepared cavity. The gutta-percha is used to fill the prepared cavity. The nitinol and gutta-percha are important contributing biomaterial for success of root canal treatment. This review article has been reviewed contemporary studies on nitinol and gutta-percha biomaterials. For the success of root canal treatment the fatigue of Endodontic file and three dimensional filling of root canal are two important aspect. The Endodontic files during the cleaning and shaping of the canal gets subjected to torsion fatigue failure or cyclic fatigue failure. The past studies lacks the exact knowledge about these failures as well as analysis approaches to be followed for correct diagnosis. This review article helps to overcome this difficulties. Moreover, the fatigue factors are discussed so that researcher can get little idea about failure point of the nitinol rotary files. The review consist of A contemporary review of nitinol alloy studies, A review of fatigue failure diagnosis, A contemporary review of gutta-percha quality assessment techniques studies, A novel approach for fatigue analysis of nitinol file and A novel approach for quality assessment of gutta-percha etc.